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HEK293 TLR Reporter Cells

InvivoGen offers a collection of HEK293-derived TLR (Toll-like receptor) reporter cells.


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HEK293 TLR Reporter cells are designed for rapid, sensitive, and reliable screening of TLR agonists or antagonists.

InvivoGen offers a series of cell lines to monitor TLR-induced NF-κB and/or IRF signaling:

– HEK-Blue™ TLR cells
– HEK-Blue-Lucia™ TLR cells
– HEK-Dual™ TLR cells

These cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end-user.
 


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HEK-Blue™ TLR cells (NF-κB)

  • Stable expression of a human or mouse TLR gene
  • Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene
     

HEK-Blue-Lucia™ TLR cells* (NF-κB/IL8)

  • Stable expression of a human or mouse TLR gene
  • Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene
  • Stable expression of the Lucia luciferase reporter gene under the control of the endogenous IL-8 promoter (responsive to NF-κB)
  • No activity of TLR3, TLR5, and TNFR (Tumor Necrosis Factor receptor) due to verified knockout of these three receptors


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​*Note: these cells were formerly named HEK-Dual™ TLR (NF/IL8) cells.
 

HEK-Dual™ TLR cells (NF-κB/IRF)

  • Stable expression of a human TLR gene
  • Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene
  • Stable expression of an IRF-inducible Lucia luciferase reporter gene

 

 

The activity of the reporter proteins, SEAP and Lucia luciferase, can be conveniently assayed in the cell supernatants using the SEAP detection reagents, QUANTI-Blue™ Solution or HEK-Blue™ Detection, and the Lucia luciferase detection reagent, QUANTI-Luc™ 4 Lucia/Gaussia.

 

InvivoGen also offers a comprehensive collection of TLR ligands and TLR inhibitors.

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