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HEK TLR Reporter Cells

InvivoGen offers a collection of HEK293-derived TLR (Toll-like receptor) reporter cells designed for rapid, sensitive, and reliable screening of TLR agonists or antagonists.

HEK-Blue™ TLR cells

  • Stable expression of a human or mouse TLR gene
  • Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene

HEK-Dual™ TLR (NF/IL8) cells

  • Stable expression of a human or mouse TLR gene
  • Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene
  • Stable expression of the Lucia luciferase reporter gene under the control of the endogenous IL-8 promoter.
  • No activity of TLR3, TLR5, and TNFR (Tumor Necrosis Factor receptor).

The activity of the reporter proteins, SEAP and Lucia luciferase, can be conveniently assayed in the cell supernatants using the SEAP detection reagents, QUANTI-Blue™ or HEK-Blue™ Detection, and the Lucia detection reagent, QUANTI-Luc™.

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