HEK293 TLR Reporter Cells

InvivoGen offers a collection of HEK293-derived TLR (Toll-like receptor) reporter cells.

HEK293 TLR Reporter cells are designed for rapid, sensitive, and reliable screening of TLR agonists or antagonists.

InvivoGen offers a series of cell lines to monitor TLR-induced NF-κB and/or IRF signaling:

– HEK-Blue™ TLR cells
– HEK-Blue-Lucia™ TLR cells
– HEK-Dual™ TLR cells

These cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end-user.

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HEK-Blue™ TLR cells (NF-κB)

Stable expression of a human or mouse TLR gene
Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene

HEK-Blue-Lucia™ TLR cells* (NF-κB/IL8)

Stable expression of a human or mouse TLR gene
Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene
Stable expression of the Lucia luciferase reporter gene under the control of the endogenous IL-8 promoter (responsive to NF-κB)
No activity of TLR3, TLR5, and TNFR (Tumor Necrosis Factor receptor) due to verified knockout of these three receptors

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*Note: these cells were formerly named HEK-Dual™ TLR (NF/IL8) cells.

HEK-Dual™ TLR cells (NF-κB/IRF)

Stable expression of a human TLR gene
Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene
Stable expression of an IRF-inducible Lucia luciferase reporter gene

The activity of the reporter proteins, SEAP and Lucia luciferase, can be conveniently assayed in the cell supernatants using the SEAP detection reagents, QUANTI-Blue™ Solution or HEK-Blue™ Detection, and the Lucia luciferase detection reagent, QUANTI-Luc™ 4 Lucia/Gaussia.

InvivoGen also offers a comprehensive collection of TLR ligands and TLR inhibitors.

Products

TLR2 reporter cells
HEK-Blue™ hTLR2		Human TLR2 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Blue™ hTLR2 KO-TLR1/6		Human TLR2 expressing, knockout for TLR1/6 & NF-κB-SEAP HEK293 reporter cells
HEK‑Blue™ hTLR2-TLR1		Human TLR2 and TLR1 expressing & NF-κB-SEAP HEK293 reporter cells
HEK‑Blue™ hTLR2-TLR6		Human TLR2 and TLR6 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Blue™ mTLR2		Murine TLR2 expressing NF-κB-SEAP HEK293 reporter cells
HEK-Blue-Lucia™ hTLR2		Human TLR2 expressing & NF-κB-SEAP and IL-8-Lucia HEK293 reporter cells
TLR3 reporter cells
HEK-Blue™ hTLR3		Human TLR3 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Blue™ mTLR3		Murine TLR3 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Blue-Lucia™ hTLR3		Human TLR3 expressing double NF-κB–readout HEK293 reporter cells
HEK-Dual™ hTLR3		Human TLR3 expressing & NF-κB-SEAP and IRF-Lucia HEK293 Reporter Cells
HEK-Dual™ RNA-hTLR3		Specific human TLR3 (RIG-I & MDA5 deficient) & NF-κB-SEAP and IRF-Lucia Reporter Cells
TLR4 / MD2-CD14 reporter cells
HEK-Blue™ hTLR4		Human TLR4 expressing NF-κB-SEAP HEK293 reporter cells
HEK-Blue™ mTLR4		Murine TLR4 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Blue™ hMD2-CD14		Human MD2 and CD14 expressing & NF-κB–SEAP HEK293 reporter cells
HEK-Blue-Lucia™ mTLR4		Murine TLR4 expressing & NF-κB-SEAP and IL-8-Lucia HEK293 reporter cells
HEK-Dual™ hTLR4		Human TLR4 expressing & NF-κB-SEAP and IRF-Lucia HEK293 Reporter Cells
TLR5 reporter cells
HEK-Blue™ hTLR5		Human TLR5 expressing NF-κB-SEAP HEK293 reporter cells
HEK-Blue™ mTLR5		Murine TLR5 expressing NF-κB-SEAP HEK293 reporter cells
HEK-Blue-Lucia™ hTLR5		Human TLR5 expressing & NF-κB-SEAP and IL-8-Lucia HEK293 reporter cells
TLR7 reporter cells
HEK-Blue™ hTLR7		Human TLR7 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Blue™ mTLR7		Murine TLR7 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Blue-Lucia™ mTLR7		Murine TLR7 expressing & NF-κB-SEAP and IL-8-Lucia HEK293 reporter cells
HEK-Dual™ hTLR7		Human TLR7 expressing & NF-κB-SEAP and IRF-Lucia HEK293 Reporter Cells
TLR8 reporter cells
HEK-Blue™ hTLR8		Human TLR8 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Blue™ mTLR8		Murine TLR8 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Dual™ hTLR8		Human TLR8 expressing & NF-κB-SEAP and IRF-Lucia HEK293 Reporter Cells
TLR9 reporter cells
HEK-Blue™ hTLR9		Human TLR9 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Blue™ mTLR9		Mouse TLR9 expressing & NF-κB-SEAP HEK293 reporter cells
HEK-Blue-Lucia™ hTLR9		Human TLR9 expressing & NF-κB-SEAP and IL-8-Lucia HEK293 reporter cells
HEK-Dual™ hTLR9		Human TLR9 expressing & NF-κB-SEAP and IRF-Lucia HEK293 Reporter Cells