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HEK TLR Reporter Cells

InvivoGen offers two series of HEK293-derived TLR (Toll-like receptor) reporter cells designed to provide a rapid, sensitive and reliable method to screen and validate TLR agonists or antagonists.

Both series allow the study of TLR stimulation by monitoring the activation of the transcription factors NF-κB and AP-1.

HEK-Blue™ TLR cells

Well-established family of TLR reporter cells

HEK-Blue™ TLR cells allow the study of TLR stimulation by monitoring the activation of the transcription factors NF-κB and AP-1. They stably co-express a TLR gene and an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene.

HEK-Dual™ TLR (NF/IL8) cells

New family of TLR reporter cells

In addition to the monitoring of TLR-induced NF-κB/AP-1 activation, HEK-Dual™ TLR (NF/IL8) cells allow the monitoring of TLR-induced IL-8 expression. They stably co-express a TLR gene, an NF-κB/AP1-inducible SEAP reporter gene, and the Lucia Luciferase reporter gene placed under the control of the endogenous IL-8 promoter (through the use of knockin technology). Furthermore, these cells have been engineered to eliminate the endogenous activity of TLR3, TLR5 and TNFR (Tumor Necrosis Factor receptor).

 

The activity of the reporter proteins, SEAP and Lucia luciferase, can be conveniently assayed in the cell supernatants using the SEAP detection reagents, QUANTI-Blue™ or HEK-Blue™ Detection, and the Lucia detection reagent, QUANTI-Luc™.

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