Sequencing Primers

Open Reading Frame Vector Sequencing Primers

pUNO / pUNO1 / pUNO2 / pUNO3 / pSelect
5' primerTGCTTGCTCAACTCTACGTCForwardHTLV 5'UTR
3' primerAACTTGTTTATTGCAGCTTReverseSV40 pAn
pLV
5' primerTGGTTACAATGATATACACTGForwardrbt ßGlo intron
3' primerCTCAAGGGGCTTCATGATGTCReverserbt ßGlo 3'UTR pAn

 

Cloning Vector Sequencing Primers

pCpGfree
5' primerGCAGGAAAAGTGGCACTGTGForwardEF1 5'UTR
3' primerAACTTGTTTATTGCAGCTTReverseSV40 pAn
pCpGfree-basic
5' primerGTACCAGTTTTATTGTTTTTAGTGGTAGTGForwardßGlobin MAR
3' primerCATGGTGGAAGCTACTGTACACReverseUTR5'
 pCpGfree-promoter
5' primerGTACCAGTTTTATTGTTTTTAGTGGTAGTGForwardßGlobin MAR
3' primerGCCATGTGCTCTCTGCCCACTGAGReverseEF1 prom
 pCpGfree-vitro
5' primerGTGCAGTAGTCTCTGTGAACATTCAAGCForwardEF1 prom
3' primerAACTTGTTTATTGCAGCTTReverseSV40 pAn
pCpGrich
5' primerGCAGGAAAAGTGGCACTATGForwardEF1 5'UTR
3' primerAACTTGTTTATTGCAGCTTReverseSV40 pAn
pFUSE-CLIg / pFUSE-CHIg
5' primerTGCTTGCTCAACTCTACGTCForwardHTLV 5'UTR
pFUSE-Fc / pINFUSE (human IgG1, IgG2, IgG3, IgG4 and rabbit IgG)
5' primerTGCTTGCTCAACTCTACGTCForwardHTLV 5'UTR
3' primerCTCACGTCCACCACCACGCAReverseFc
pFUSE-Fc (mouse IgG1)
5' primerTGCTTGCTCAACTCTACGTCForwardHTLV 5'UTR
3' primerGTGAGCACATCCTTGGGCTTReverseFc
pFUSE-Fc / pINFUSE (mouse IgG2a, IgG2b, IgG3)
5' primerTGCTTGCTCAACTCTACGTCForwardHTLV 5'UTR
3' primerGGAAGATGAAGACGGATGGTCReverseFc
pFUSE-Fc (rat IgG1)
5' primerTGCTTGCTCAACTCTACGTCForwardHTLV 5'UTR
3' primerTGGGAGATCAAGAGGATGTCReverseFc
pFUSEN   
3' primerAACTTGTTTATTGCAGCTTReverseSV40 pAn
    
pTRIOZ
Light chain, 5’ primerTTGCCTCATTCTCAAGCCTCAGACForwardchEF1 5'UTR
Light chain, 3’ primerAACTTGTTTATTGCAGCTTReverseSV40 pAn
Heavy chain, 5’ primerGGGGGAGGGGATGTAATGGCGTTGForwardmEF1 5'UTR
Heavy chain, 3’ primerTTAGGCAGAATCCAGATGCTCReversehßGlobin pAn
    
pVAC1
5' primerTGTATAGGATGCAACTGCTGForwardIL2ss
3' primerGAAACAAACAGTTCTGAGACCGReverseEF1 pAn
pVAC2
5' primerTCTCAAGCTTCAGACAGTGForwardrhEF1 5'UTR
3' primerGAAACAAACAGTTCTGAGACCGReverseEF1 pAn
    
pVITRO1
5' primerGAGGCTAATTCTCAAGCCTCMCS1 ForwardrEF1 5'UTR
3' primerTCTAGACCTGGAAAGACCAGMCS1 ReverseFMDV IRES
5' primerTTTTGAGCGGAGCTAATTCTCGGGMCS2 ForwardmEF1 5'UTR
3' primerAAAAAACCTCCCACACCTCCMCS2 ReverseSV40 pAn
pVITRO2 / pDUO2
5' primerTTTTGAGCGGAGCTAATTCTCGGGMCS1 ForwardmEF1 5'UTR
3' primerTCTAGACCTGGAAAGACCAGMCS1 ReverseFMDV IRES
5' primerTTGCCTCATTCTCAAGCCTCAGACMCS2 ForwardchEF1 5'UTR
3' primerGCATTCTAGTTGTGGTTTGTCCMCS2 ReverseSV40 pAn
    
pVIVO1
5' primerGCGCGGCTGGAGGTGTGAGGMCS1 ForwardhGRP94 prom
3' primerGAAACAAACAGTTCTGAGACCMCS1 ReverseEF1 pAn
5' primerCTCCTGTCGCCCTCAGATCGMCS2 ForwardhGRP78 prom
3' primerAAAAAACCTCCCACACCTCCMCS2 ReverseSV40 pAn
pVIVO2
5' primerTTTTGAGCGGAGCTAATTCTCGGGMCS1 ForwardmEF1 5'UTR
3' primerGAAACAAACAGTTCTGAGACCGMCS1 ReverseEF1 pAn
5' primerTTGCCTCATTCTCAAGCCTCAGACMCS2 ForwardchEF1 5'UTR
3' primerGCATTCTAGTTGTGGTTTGTCCMCS2 ReverseSV40 pAn
    
pMONO
5' primerTTTTGAGCGGAGCTAATTCTCGGGMCS ForwardmEF1 5'UTR
3' primerTCTAGACCTGGAAAGACCAGMCS ReverseFMDV IRES

 

Discontinued vectors

pBLAST42 / pBLAST49 / pORF / pORF9
5' primerTGCTTGCTCAACTCTACGTCForwardHTLV 5'UTR
3' primerAACTTGTTTATTGCAGCTTReverseSV40 pAn
pBLAST45 / pORF5
5' primerAGCTTTCTTTCCCCAGATCCForwardeiF4g 5'UTR
3' primerAACTTGTTTATTGCAGCTTReverseSV40 pAn