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Spike Pseudotyping Vectors

SARS-CoV-2 spike (S)-pseudotyped lentiviral particle production
Production of SARS-CoV-2 spike (S)-pseudotyped lentiviral particles using InvivoGen's pLV-Spike plasmids

Original (D614) and variants of concern

InvivoGen offers a collection of plasmids specifically designed for pseudotyping lentiviral particles with the full-length SARS-CoV-2 Spike (S) protein. For optimal expression,  the spike gene has been codon-optimized and the C‑terminal ER-retention signal has been removed.

 

Various SARS-CoV-2 variants are available:

 

Click here for more information on the emerging SARS-CoV-2 Variants

 

Application

Pseudotyped particle production involves the co-transfection of 293T cells with a reporter protein vector (e.g. GFP), one or several plasmids encoding the necessary lentiviral proteins, and InvivoGen's pseudotyping pLV-Spike plasmids. The transfected cells produce SARS‑CoV‑2 Spike (S)‑pseudotyped lentiviral particles, which can then be purified and used to infect permissive cells, such as ACE2‑expressing HEK293-derived cells and ACE2‑TMPRSS2‑expressing A549-derived cells.

 

 

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