Spike Pseudotyping Vectors
InvivoGen also offers:
Original Wuhan-Hu-1 and variants of concern
InvivoGen offers a collection of plasmids specifically designed for pseudotyping lentiviral particles with the full-length SARS-CoV-2 Spike (S) protein. For optimal expression, the spike gene has been codon-optimized and the C‑terminal ER-retention signal has been removed.
Various SARS-CoV-2 variants are available:
- Wuhan-Hu-1 (clade 19)
- G614-variant (clade 20)
- United Kingdom B.1.1.7 variant (clade 20I)
- South African B.1.351 variant (clade 20H)
- Californian B.1.429 variant (clade 20C)
More variants coming soon…
Click here for more information on the emerging SARS-CoV-2 Variants
Pseudotyped particle production involves the co-transfection of 293T cells with a reporter protein vector (e.g. GFP), one or several plasmids encoding the necessary lentiviral proteins, and InvivoGen's pseudotyping pLV-Spike plasmids. The transfected cells produce SARS‑CoV‑2 Spike (S)‑pseudotyped lentiviral particles, which can then be purified and used to infect permissive cells, such as ACE2‑expressing HEK293-derived cells and ACE2‑TMPRSS2‑expressing A549-derived cells.