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Immunomodulatory Compound Screening

Compound Profiling Unit size Cat. code $ £ Order
Single dose testing on a set of PRRs
1 test tlrl-test1 From 1260€ From $1100 From £1155 Ask for a quote

 

Compound Dose Response Unit size Cat. code $ £ Order
Dose response on one or several PRRs
1 test tlrl-test2 From 800€ From $1100 From £600 Ask for a quote

 

Simplified representation of the TLR, NOD and RLR pathways
Simplified representation of the TLR, NOD and RLR pathways

PRR Ligand Screening Service

InvivoGen can screen your compounds to identify potent activators or inhibitors of innate immune signaling pathways involved in infectious diseases, auto-immunity and cancer:

  • TLR Ligand Screening

  • NOD1/2 Ligand Screening

  • STING Ligand Screening

  • Dectin-1 Ligand Screening

  • Mincle Ligand Screening

 

InvivoGen has developed a large array of functional whole-cell assays to screen for compounds that activate or inhibit pattern recognition receptors (PRRs), with an emphasis on TLRs, NOD1/2, C-type lectins and STING.

These sensitive assays employ engineered HEK293 cells that utilize classical reporter systems: an NF-kB-inducible SEAP (secreted embryonic alkaline phosphatase) and/or an IRF (interferon regulatory factor)-inducible sLUC (secreted luciferase).
Activation of the NF-kB and/or IRF pathways by the test compounds is detected using InvivoGen's proprietary methods, which we have designed for its reliability.

Figures

Example of Level 1 Compound Profiling
Example of Level 1 Compound Profiling

TLR Response Profile.
Engineered HEK-Blue™ reporter cells were stimulated with 1/10 dilution of the sample solution provided and a fixed concentration of the positive controls.
After 24h incubation, TLR-induced NF-κB activation was assessed by measuring the levels of SEAP in the supernatants of HEK-Blue™ cells using QUANTI-Blue™.

Example of Level 2 Compound Dose Response
Example of Level 2 Compound Dose Response

Dose Response on hTLR7.
Engineered HEK-Blue™ reporter cells were stimulated with 1/10, 1/30, 1/100 dilution of the sample solution provided and different concentration of the positive control (R848).
After 24h incubation, TLR induced NF-κB activation was assessed by measuring the levels of SEAP in the supernatants of HEK-Blue™ cells using QUANTI-Blue™.

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Screening Specifications

Short turnaround time Short turnaround time - Screening turnaround: ONLY 3 weeks

Screening flexibility Screening flexibility - Screening parameters can be selected and/or modified based on customer requirements.

Cost effective Cost effective - A set-up charge applies for the first compound. Subsequent compounds are heavily discounted.

Reliable Reliable - Our screening service has been utilized consistently by leading Biotech and Pharmaceutical companies and academic institutes for many years.

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Screening Services

Two choices of services are offered that can be performed sequentially or individually :

Level 1 - Compound Profiling: Single dose testing on a set of PRRs.

Screening is performed at a single concentration, typically a 1/10 dilution of the original compound solution provided, or customer specified.

 

Level 2 - Compound Dose Response: Dose response on one or several PRRs.

Three concentrations of the compound(s), typically 1/10, 1/100 and 1/1000 dilutions of the original compound solution, are tested on the PRR(s) recognizing the compound(s) as determined in level 1 or specified by the customer.

 

✔ A detailed report is prepared and provided to the customer electronically and in hard copy.

✔ All procedures are performed accordingly to strict guidelines.

✔ Confidentiality is guaranteed.

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Description

TLR Ligand Screening

The TLR ligand screening service utilizes the HEK-Blue TLR cells.
These HEK293-derived cells are NF-kB-SEAP repor ter cells that stably express a human or mouse TLR gene. The TLR genes expressed are TLR2, 3, 4, 5, 7, 8, and 9.
Compound profiling: The compound is tested on all human and/or mouse TLRs to determine which TLR recognizes this compound. TLR ligands are typically recognized by a single TLR or potentially two (TLR7 and TLR8). Recognition by TLR4 usually reflects the presence of endotoxins in the sample.
Agonist and antagonist assays can be performed.

NOD1/2 Ligand Screening

The NOD1/2 ligand screening service utilizes the HEK-Blue NOD cells.
These HEK-Blue cells stably express an NF-κB-inducible SEAP reporter gene and the human or mouse NOD1 or NOD2 gene.
The compound can be tested on human and/or mouse NOD1 and NOD2.

Dectin-1 Ligand Screening

The Dectin-1 ligand screening service utilizes the HEK-Blue™ Dectin-1 cells.
These cell lines express different isoforms of the Dectin-1 gene. They also express genes of the Dectin-1-NF-κB signalling pathway, in addition to an NF-κB-inducible SEAP reporter gene.
The coumpound can be tested on human Dectin-1a, human Dectin-1b and/or mouse Dectin-1a.
Agonist and antagonists assays can be performed.

Mincle Ligand Screening

The Mincle ligand screening service utilizes the HEK-Blue™ hMincle cell line.
This cell co-expresses the human Mincle gene, genes of the Mincle-NF-κB signaling pathway and an NF-κB-inducible SEAP reporter gene.Agonist and antagonists assays can be performed, although as of today no ligand with inhibitory activity on Mincle has been identified.

 

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