Immunomodulatory Compound Screening
PRR Ligand Screening Service
InvivoGen can screen your compounds to identify potent activators or inhibitors of innate immune signaling pathways involved in infectious diseases, auto-immunity and cancer:
- TLR Ligand Screening
- NOD1/2 Ligand Screening
- STING Ligand Screening
- RIG-I/MDA5 Ligand Screening
- Dectin-1 Ligand Screening
- Mincle Ligand Screening
We have developed a large array of functional whole-cell assays to screen for compounds that activate or inhibit pattern recognition receptors (PRRs), with an emphasis on TLRs, NOD1/2, RIG-I/MDA-5, C-type lectins and STING.
These sensitive assays employ engineered HEK293 cells that utilize classical reporter systems: an NF-kB-inducible SEAP (secreted embryonic alkaline phosphatase) and/or an IRF (interferon regulatory factor)-inducible sLUC (secreted luciferase). Activation of the NF-kB and/or IRF pathways by the test compounds is detected using InvivoGen's proprietary methods, which we have designed for its reliability.
|Compound Profiling||Unit size||Cat. code||€||$||£||Order|
Single dose testing on a set of PRRs
|1 test||tlrl-test1||From 800€||From $1100||From £600||Ask for a quote|
|Compound Dose Response||Unit size||Cat. code||€||$||£||Order|
Dose response on one or several PRRs
|1 test||tlrl-test2||From 800€||From $1100||From £600||Ask for a quote|
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Short turnaround time - Screening turnaround: ONLY 3 weeks
Screening flexibility - Screening parameters can be selected and/or modified based on customer requirements.
Cost effective - A set-up charge applies for the first compound. Subsequent compounds are heavily discounted.
Reliable - Our screening service has been utilized consistently by leading Biotech and Pharmaceutical companies and academic institutes for many years.Back to the top
Two choices of services are offered that can be performed sequentially or individually :
• Compound Profiling (level 1): Single dose testing on a set of PRRs.
Screening is performed at a single concentration, typically a 1/10 dilution of the original compound solution provided, or customer specified.
• Compound Dose Response (level 2): Dose response on one or several PRRs
Three concentrations of the compound(s), typically 1/10, 1/100 and 1/1000 dilutions of the original compound solution, are tested on the PRR(s) recognizing the compound(s) as determined in level 1 or specified by the customer.
A detailed report is prepared and provided to the customer electronically and in hard copy. All procedures are performed accordingly to strict guidelines. Confidentiality is guaranteed.Back to the top
TLR Ligand Screening
The TLR ligand screening service utilizes the HEK-Blue TLR cells.
These HEK293-derived cells are NF-kB-SEAP repor ter cells that stably express a human or mouse TLR gene. The TLR genes expressed are TLR2, 3, 4, 5, 7, 8, and 9.
Compound profiling: The compound is tested on all human and/or mouse TLRs to determine which TLR recognizes this compound. TLR ligands are typically recognized by a single TLR or potentially two (TLR7 and TLR8). Recognition by TLR4 usually reflects the presence of endotoxins in the sample.
Agonist and antagonist assays can be performed.
NOD1/2 Ligand Screening
The NOD1/2 ligand screening service utilizes the HEK-Blue NOD cells.
These HEK-Blue cells stably express an NF-κB-inducible SEAP reporter gene and the human or mouse NOD1 or NOD2 gene.
The compound can be tested on human and/or mouse NOD1 and NOD2.
RIG-I/MDA-5 Ligand Screening
The RIG-I/MDA-5 ligand screening service utilizes HEK293 cell lines that exploit the IRF pathway and a secreted luciferase reporter assay. They overexpress the human RIG-I or MDA-5 gene and are highly sensitive to 5’ppp-dsRNA and transfected poly(I:C), respectively, unlike their parental cell line that expresses low levels of both RIG-I and MDA-5.
The compound can be tested on human RIG-I and/or MDA-5.
Dectin-1 Ligand Screening
The Dectin-1 ligand screening service utilizes the HEK-Blue™ Dectin-1 cells.
These cell lines express different isoforms of the Dectin-1 gene. They also express genes of the Dectin-1-NF-κB signalling pathway, in addition to an NF-κB-inducible SEAP reporter gene.
The coumpound can be tested on human Dectin-1a, human Dectin-1b and/or mouse Dectin-1a.
Agonist and antagonists assays can be performed.
Mincle Ligand Screening
The Mincle ligand screening service utilizes the HEK-Blue™ hMincle cell line.
This cell co-expresses the human Mincle gene, genes of the Mincle-NF-κB signaling pathway and an NF-κB-inducible SEAP reporter gene.Agonist and antagonists assays can be performed, although as of today no ligand with inhibitory activity on Mincle has been identified.
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Simplified representation of the TLR, NOD and RLR pathwaysBack to the top