Immunomodulatory Compound Screening

Compound Profiling Unit size Cat. code $ £ Order
Single dose testing on a set of PRRs
1 test tlrl-test1 From 1260€ From $1100 From £1155 Ask for a quote


Compound Dose Response Unit size Cat. code $ £ Order
Dose response on one or several PRRs
1 test tlrl-test2 From 800€ From $1100 From £600 Ask for a quote


PRR Ligand Screening Service

InvivoGen can screen your compounds to identify potent activators or inhibitors of innate immune signaling pathways involved in infectious diseases, auto-immunity and cancer:

  • TLR Ligand Screening
  • NOD1/2 Ligand Screening
  • STING Ligand Screening
  • RIG-I/MDA5 Ligand Screening
  • Dectin-1 Ligand Screening
  • Mincle Ligand Screening

We have developed a large array of functional whole-cell assays to screen for compounds that activate or inhibit pattern recognition receptors (PRRs), with an emphasis on TLRs, NOD1/2, RIG-I/MDA-5, C-type lectins and STING.

These sensitive assays employ engineered HEK293 cells that utilize classical reporter systems: an NF-kB-inducible SEAP (secreted embryonic alkaline phosphatase) and/or an IRF (interferon regulatory factor)-inducible sLUC (secreted luciferase). Activation of the NF-kB and/or IRF pathways by the test compounds is detected using InvivoGen's proprietary methods, which we have designed for its reliability.

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Short turnaround time - Screening turnaround: ONLY 3 weeks

Screening flexibility - Screening parameters can be selected and/or modified based on customer requirements.

Cost effective - A set-up charge applies for the first compound. Subsequent compounds are heavily discounted.

Reliable - Our screening service has been utilized consistently by leading Biotech and Pharmaceutical companies and academic institutes for many years.

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Screening Services

Two choices of services are offered that can be performed sequentially or individually :

• Compound Profiling (level 1): Single dose testing on a set of PRRs.
Screening is performed at a single concentration, typically a 1/10 dilution of the original compound solution provided, or customer specified.

• Compound Dose Response (level 2): Dose response on one or several PRRs
Three concentrations of the compound(s), typically 1/10, 1/100 and 1/1000 dilutions of the original compound solution, are tested on the PRR(s) recognizing the compound(s) as determined in level 1 or specified by the customer.

A detailed report is prepared and provided to the customer electronically and in hard copy. All procedures are performed accordingly to strict guidelines. Confidentiality is guaranteed.

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TLR Ligand Screening

The TLR ligand screening service utilizes the HEK-Blue TLR cells.
These HEK293-derived cells are NF-kB-SEAP repor ter cells that stably express a human or mouse TLR gene. The TLR genes expressed are TLR2, 3, 4, 5, 7, 8, and 9.
Compound profiling: The compound is tested on all human and/or mouse TLRs to determine which TLR recognizes this compound. TLR ligands are typically recognized by a single TLR or potentially two (TLR7 and TLR8). Recognition by TLR4 usually reflects the presence of endotoxins in the sample.
Agonist and antagonist assays can be performed.

NOD1/2 Ligand Screening

The NOD1/2 ligand screening service utilizes the HEK-Blue NOD cells.
These HEK-Blue cells stably express an NF-κB-inducible SEAP reporter gene and the human or mouse NOD1 or NOD2 gene.
The compound can be tested on human and/or mouse NOD1 and NOD2.

RIG-I/MDA-5 Ligand Screening

The RIG-I/MDA-5 ligand screening service utilizes HEK293 cell lines that exploit the IRF pathway and a secreted luciferase reporter assay. They overexpress the human RIG-I or MDA-5 gene and are highly sensitive to 5’ppp-dsRNA and transfected poly(I:C), respectively, unlike their parental cell line that expresses low levels of both RIG-I and MDA-5.
The compound can be tested on human RIG-I and/or MDA-5.

Dectin-1 Ligand Screening

The Dectin-1 ligand screening service utilizes the HEK-Blue™ Dectin-1 cells.
These cell lines express different isoforms of the Dectin-1 gene. They also express genes of the Dectin-1-NF-κB signalling pathway, in addition to an NF-κB-inducible SEAP reporter gene.
The coumpound can be tested on human Dectin-1a, human Dectin-1b and/or mouse Dectin-1a.
Agonist and antagonists assays can be performed.

Mincle Ligand Screening

The Mincle ligand screening service utilizes the HEK-Blue™ hMincle cell line.
This cell co-expresses the human Mincle gene, genes of the Mincle-NF-κB signaling pathway and an NF-κB-inducible SEAP reporter gene.Agonist and antagonists assays can be performed, although as of today no ligand with inhibitory activity on Mincle has been identified.


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Simplified representation of the TLR, NOD and RLR pathways

Simplified representation of the TLR, NOD and RLR pathways

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