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Pam3CSK4

Pam3CSK4 Unit size Cat. code Docs Qty Price
Synthetic triacylated lipopeptide - TLR1/2
1 mg
tlrl-pms
+-
$147.00

Synthetic triacylated lipopeptide

Pam3CSK4 is a synthetic triacylated lipopeptide (LP) that mimics the acylated amino terminus of bacterial LPs.

Pam3CSK4 is a potent activator of the proinflammatory transcription factor NF-κB.

Activation is mediated by the TLR2/TLR1 heterodimer, which recognizes LPs with three fatty acids, a structural characteristic of bacterial LPs.

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Specifications

Specificity: TLR 1/2 agonist

Working concentration: 1 - 300 ng/ml

CAS number:112208-00-1

Molecular formula: C81H156N10O13S

Molecular weight: 1510.24

Solubility: 2 mg/ml in water

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Contents

Pam3CSK4 is provided lyophilized.

  • tlrl-pms: 1 mg Pam3CSK4 + 1.5 ml endotoxin-free water

room temperature Pam3CSK4 is shipped at room temperature.

Storage Upon receipt it should be stored at 4°C.

stability Lyophilized Pam3CSK4 is stable 1 year when properly stored.

Upon resuspension, prepare aliquots of Pam3CSK4 and store at 4°C for short term storage or -20°C for long storage.

Resuspended product is stable 1 month at 4°C and 6 months at -20°C when properly stored.

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Description

Bacterial lipoproteins are a family of proinflammatory cell wall components found in both Gram positive and Gram negative bacteria.

The stimulatory activity of bacterial lipoproteins resides in their acylated amino terminus. Pam3CSK4 is a synthetic tripalmitoylated lipopeptide that mimics the acylated amino terminus of bacterial lipoproteins.

Pam3CysSerLys4 (Pam3CSK4) is a potent activator of the proinflammatory transcription factor NF-κB [1].

Recognition of Pam3CSK4 is mediated by TLR2 which cooperates with TLR1 through their cytoplasmic domain to induce the signaling cascade leading to the activation of NF-κB [2].
 

1. Aliprantis AO et al., 1999. Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2. Science.285(5428):736-9.
2. Ozinsky A. et al., 2000. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. PNAS. 97(25):13766-71.

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Details

Chemical structure of Pam3CSK4

Chemical structure of Pam3CSK4

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Citations

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