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Synthetic diacylated lipoprotein - TLR2/6
Synthetic diacylated lipoprotein
FSL-1 (Pam2CGDPKHPKSF) is a synthetic lipopeptide derived from Mycoplasma salivarium. It contains the same diacylglycerol structure as MALP-2 from M. fermentans but has a different peptide sequence.
The mycoplasmal lipopeptide FSL-1 contains a diacylated cysteine residue and is recognized by the TLR2/TLR6 heterodimer.
Specificity: TLR2/6 agonist
Working concentration: 1 - 100 ng/ml
Solubility: 10 mg/ml in water
Endotoxin level: Molecular weight: 1666.2
Molecular formula: C84H140N14O18S
• 100 μg lipopeptide FSL-1
• 1.5 ml sterile endotoxin-free water
FSL-1 is provided lyophilized and shipped at room temperature. Store at 4 ̊C.Back to the top
FSL-1 (Pam2CGDPKHPKSF) is a synthetic lipoprotein that represents the N-terminal part of the 44-kDa lipoprotein LP44 of Mycoplasma salivarium . The framework structure of FSL-1 is the same as that of MALP-2, a Mycoplasma fermentans derived lipopeptide (LP), but they differ in the amino acid sequence and length of the peptide portion . Mycoplasmal LP, such as FSL-1 and MALP-2, contain a lipolyated N-terminal diacylated cysteine residue, whereas bacterial LP contain a triacylated one. This structural difference plays a crucial role in the initial recognition of microbial LP by the host innate immune system. Mycoplasmal LP, such as FSL-1, are recognized by TLR2 and TLR6, whereas bacterial LP and Pam3CSK4, a synthetic LP, are recognized by TLR2 and TLR1 . FSL-1 stimulation induces a MyD88-dependent signaling cascade leading to the activation of NF-κB and the production of proinflammatory cytokines. FSL-1 exhibits a higher stimulatory activity than MALP2 when tested for its ability to induce the production of TNFa in macrophages and NF-κB in TLR2/TLR6 transfected HEK293 cells.
1. Shibata, KI. et al., 2000. The N-terminal lipopeptide of a 44-kDa membrane-bound lipoprotein of Mycoplasma salivarium is responsible for the expression of intercellular adhesion molecule-1 on the cell surface of normal human gingival fibroblasts. J. Immunol. 165:6538–6544.
2. Okusawa T. et al., 2004. Relationship between Structures and Biological Activities of Mycoplasmal Diacylated Lipopeptides and Their Recognition by Toll-Like Receptors 2 and 6. Infect Immun. 72(3): 1657-1665.
3. Takeuchi O. et al., 2001. Discrimination of bacterial lipoproteins by Toll-like receptor 6. Int Immunol. 13(7):933-40.