Human TLR4 Reporter HEK293 Cells (NF-κB)
HEK-Blue™ hTLR4 cells | Unit size | Cat. code | Docs | Qty | Price |
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Human TLR4 expressing HEK293 reporter cells (NF-κB pathway)
|
3-7 x 10e6 cells |
hkb-htlr4 |
You may also need : HEK-Blue™ Selection | View more associated products ▼
Notification: This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.
NF-κB–SEAP reporter HEK293 cells expressing human TLR4
HEK-Blue™-hTLR4 cells were obtained by co-transfection of the human TLR4, MD-2 and CD14 co-receptor genes, and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP reporter gene is placed under the control of an IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites.
Stimulation with a TLR4 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR4 cells is HEK-Blue™ Null2 cells.
Back to the topSpecifications
Antibiotic resistance: blasticidin, hygromycin, Zeocin®
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free.
This product is covered by a Limited Use License (See Terms and Conditions).
Back to the topContents
1 vial containing 3-7 x 106 cells
- 2 ml 250X HEK-Blue™ Selection
- 1 ml Normocin™ (50 mg/ml)
- 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)
Shipped on dry ice. (Europe, USA, Canada and some areas in Asia)