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Blasticidin

Blasticidin (solution) Unit size Cat. code Docs Qty Price
Selective antibiotic for the bsr, bls, or BSD genes
50 mg (5 x 1 ml)
100 mg (10 x 1 ml)
500 mg (50 ml bottle)
500 mg (50 x 1 ml)
ant-bl-05
+-
$150.00
Blasticidin (powder) Unit size Cat. code Docs Qty Price
Selective antibiotic for the bsr, bls, or BSD genes
1 g
ant-bl-10p
+-
$1,423.00

Selective antibiotic | Blasticidin | InvivoGen

Selection antibiotic: cell culture tested, sterile reagent

Blasticidin is an efficient selection antibiotic that acts on both eukaryotic and prokaryotic cells

Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery.

Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase [1].
Resistance to blasticidin is also conferred by the blastcidin S acetyltransferase gene (bls) from Streptoverticillum sp [2], and the blasticidin S deaminase gene (BSD) from Apergillus terreus [3].

Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml.
Cell death induced by blasticidin occurs rapidly, allowing for selection of transfected cell lines carrying a blasticidin resistance gene within one week. 

 

1. Izumi M. et al., 1991. Blasticidin S-resistance gene (bsr): A novel selectable marker for mammalian cells. Exp.Cell Res.197:229-33.
2. Perez-Gonalez J. et al., 1990. Cloning and characterization of the gene encoding a blasticidin S acetyltransferase from Streptoverticillum sp. Gene. 86:129-34. 
3. Kimura M. et al., 1994. Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. Biochim. Biophys. Acta. 1219:653-9.

 

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Specifications

Product concentration: 10 mg/ml

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.

Purity: ≥ 95% (HPLC)

Endotoxin level: < 1 EU/mg

Physicochemical characterization: pH, appearance

Cell-culture tested: potency validated in blasticidin-sensitive and blasticidin-resistant mammalian cell lines

Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in blasticidin-resistant cells

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Contents

Blasticidin hydrochloride is supplied as a sterile filtered solution at 10 mg/ml in HEPES buffer.

This product is available in four pack sizes:

  • ant-bl-05: 5 x 1 ml (50 mg)
  • ant-bl-1: 10 x 1 ml (100 mg)
  • ant-bl-5: 50 x 1 ml (500 mg)
  • ant-bl-5b: 1 x 50ml (500 mg)

Blasticidin hydrochloride is also supplied as a powder:

  • ant-bl-10p: 1 g (powder)

Blasticidin is shipped at room temperature.
Upon receipt it should be stored at 4°C or -20°C.
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

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Citations

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