HEK-Blue™ CLR Selection

Use of selective antibiotics with HEK-Blue™ cells

Antibiotics for maintenance of HEK-Blue™ Dectin & Mincle cells

HEK-Blue™ CLR Selection is a solution that combines several selective antibiotics.

These antibiotics guarantee the persistent expression of the various transgenes introduced in HEK-Blue™ cells such as the C-type Lectin (CLR) genes, namely Dectin-1, Dectin-2, and Mincle. The use of HEK-Blue™ CLR Selection is also recommended to guarantee persistent transgene expression in HEK-Blue™ IL-2 cells.  HEK-Blue™ CLR Selection should be used for the maintenance of the cell lines listed below.

Key features of HEK-Blue™ CLR Selection

➔ Convenient

1 single tube that combines several selective antibiotics.

➔ Simple

Add directly to the culture at a 1:250 dilution.

➔ Optimized concentration

The concentration of antibiotics has been optimized to maximize their effectiveness for the maintenance of these cells.

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Specifications

Quality Control: Each lot of HEK-Blue™ CLR Selection is thoroughly tested to ensure the absence of lot-to-lot variation.

HEK-Blue™ CLR Selection should be used with the following cell lines:

• HEK-Blue™ hDectin-1a cells
• HEK-Blue™ hDectin-1b cells
• HEK-Blue™ mDectin-1b cells
• HEK-Blue™ mDectin-2 cells
• HEK-Blue™ mMincle cells
• HEK-Blue™ IL-2 cells

Contents

HEK-Blue™ CLR Selection is provided as 10 x 1 ml tubes of 250X solution.

HEK-Blue™ CLR Selection is shipped at room temperature.

Upon receipt, it should be stored at 4 °C or -20 °C.

Avoid repeated freeze-thaw cycles.