Collection of monoclonal antibodies targeting Mincle and Dectin
InvivoGen offers monoclonal antibodies (mAbs) targeting human and mouse C-type lectin receptors (CLRs). CLRs comprise a large family of receptors recognizing a wide range of microbial and fungal carbohydrates and lipids. Thus, CLRs play a pivotal role in anti-microbe immunity.
The provided family of mAbs targets the human or murine Dectin‑1, Dectin-2, or Mincle receptors. They are produced in hybridoma cells, purified by affinity chromatography, and can be used for neutralization assays and/or flow cytometry.
Key features
- Targets the human or murine Dectin-1, Dectin-2, or Mincle receptors
- Each lot is functionally tested
- Guaranteed free of bacterial contaminants
Application
- Neutralization assay
- Flow cytometry
Background
Dendritic cell-associated C-type lectin-1 (Dectin-1) is a special receptor encoded by the C-type lectin domain family 7 member A (CLEC7A) gene. Dectin-1 is one member of the C-type lectin receptors (CLRs) and is expressed predominantly on myeloid cells including dendritic cells (DCs), macrophages, neutrophils, T cells, and B cells [1-2]. Several endogenous and exogenous ligands have been reported for Dectin-1, but this receptor is best known for its ability to recognize fungal β-glucans. β-glucans are carbohydrate PAMPs found predominantly in fungal cell walls, including the yeasts Saccharomyces cerevisiae and Candida albicans [2].
Dendritic cell-associated C-type lectin-2 (Dectin-2) is another member of the CLRs and part of the Dectin-2 family. It is encoded by the C-type lectin domain containing 6A (CLEC6A) gene and is mainly expressed in tissue macrophages, DCs, monocytes, and neutrophils, as well as on Langerhans cells [3]. Dectin-2 is a receptor of high mannose structures of different microbes, including Candida albicans, Saccharomyces cerevisiae, and Mycobacterium tuberculosis[3].
Macrophage-inducible C-type lectin (Mincle), a CLR and member of the Dectin-2 family, is encoded by the C-type lectin domain family 4 member E (CLEC4E) gene and recognizes fungi and induces inflammatory signals [3]. In addition to fungi detection, Mincle can also mediate inflammatory responses to necrotic cell death, and its expression is strongly induced in response to several inflammatory stimuli and stresses, [3]. Thus, it is involved in various diseases characterized by increased inflammation, including obesity, rheumatoid arthritis, allergic contact dermatitis, and hepatitis [4].
References:
1. Kalia N, Singh J, Kaur M., 2022. The role of dectin-1 in health and disease. Immunobiology.;226(2):152071.
2. Marakalala MJ, et al., 2011. Dectin-1: a role in antifungal defense and consequences of genetic polymorphisms in humans. Mamm Genome.(1-2):55-65.
3. Graham LM, Brown GD., 2009. The Dectin-2 family of C-type lectins in immunity and homeostasis. Cytokine. ;48(1-2):148-55.
4. Lim R, Lappas M., 2019. Expression and function of macrophage-inducible C-type lectin (Mincle) in inflammation-driven parturition in fetal membranes and myometrium. Clin Exp Immunol. 197(1):95-110.
