Anti-hDectin-2-IgG
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Cat.code:
mabg-hdect2-2
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ABOUT
Human Dectin-2 neutralizing and detection antibody - Monoclonal mouse IgG2a
Anti-hDectin-2-IgG (clone Q7-4B5) is a monoclonal mouse IgG2a antibody against human Dectin-2 (hDectin-2). This antibody was screened for neutralization activity and flow cytometry. It is produced in hybridomas and purified by affinity chromatography with protein G.
Dendritic cell-associated C-type lectin-2 (Dectin-2) is a C-type lectin receptor (CLR), encoded by the C-type lectin domain containing 6A (CLEC6A) gene. It plays an important role in antifungal innate immunity [1, 2]. It binds high mannose-type carbohydrates and has been demonstrated to be the functional receptor for α-mannans.
Key features:
- Targets human Dectin-2
- Provided azide-free
- Each lot is functionally tested
Read our review on C-Type Lectin Receptors
References:
1. Hou H. et al., 2017. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 2017;13(6):536-543.
2. Wang H.L. et al., 2017. Ligation of Dectin-2 with a novel microbial ligand promotes adjuvant activity for vaccination. PLoS Pathog. 213(8):e1006568.
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Human Dectin-2 (hDectin-2)
Human
Neutralization/block, Flow cytometry
Sodium phosphate buffer with saccharose, glycine, and stabilizing agents
0.2 µm filtration
Neutralization/block, Flow cytometry
Validated for neutralization using cellular assays, Binding to hDectin-2 on cells validated using flow cytometry, Absence of bacterial contamination (e.g. lipoproteins and endotoxins) confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells
CONTENTS
Contents
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Product:Anti-hDectin-2-IgG
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Cat code:mabg-hdect2-2
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Quantity:2 x 100 µg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Dectin-2 is a member of the C-type lectin receptor (CLR) family and plays an important role in antifungal innate immunity [1].
CLRs comprise a large family of receptors that bind to carbohydrates in a calcium-dependent manner. The lectin activity of these receptors is mediated by conserved carbohydrate-recognition domains (CRDs).
Dectin-2 is a type II transmembrane CLRs with a single CRD domain. Dectin-2 binds high mannose-type carbohydrates and was shown to be the functional receptor for a-mannans. Upon binding to its ligand, Dectin-2 signals through the kinase Syk and the adaptors CARD9/Bcl-10/MALT1 triggering the activation of NF-kB and the subsequent production of pro-inflammatory cytokines [1, 2].
Dectin-2 is implicated in anti-bacterial immunity and allergy [3]. In fact, it has been demonstrated that Dectin-2 is important for the induction of Th17 in response to fungal infection [4].
References
1. Sancho D & Reis e Sousa C., 2012. Signaling by myeloid C-type lectin receptors in immunity and homeostasis. Annu Rev Immunol. 30:491-529.
2. Hernández-Santos N. & Gaffen SL., 2012. Th17 cells in immunity to Candida albicans. Cell Host Microbe. 11(5):425-35.
3. Drummond R. et al., 2011. The role of Syk/CARD9 coupled C-type lectins in antifungal immunity. Eur J Immunol. 41:276-81.
4. Saijo S. et al., 2010. Dectin-2 recognition of alpha-mannans and induction of Th17 cell differentiation is essential for host defense against Candida albicans. Immunity. 32(5):681-91.
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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