Human Dectin-1 (CLEC7A) Antibody

Mouse IgG1 (clone 22H8) - Recombinant 

ABOUT

Recombinant anti-human Dectin-1 neutralizing and detection antibody

Anti-hDectin-1-mIgG1 is a monoclonal antibody (mAb) targeting human Dectin-1. This antibody was screened for neutralization activity and flow cytometry (see figures). Anti-hDectin-1-mIgG1 was previously extracted from hybridoma cells (clone 22H8). It is now expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Anti-hDectin-1-mIgG1 recognizes hDectin-1 isoforms A and B. 

This antibody can be used together with HEK-Blue™ hDectin-1b Cells for screening and neutralization assays to block hDectin-1 signaling in response to stimulation by Dectin ligands.

 

Key features

  • Each lot is functionally tested and validated.
  • The complete sequence of the antibody construct has been verified.
  • The absence of endotoxins is determined by the EndotoxDetect™ assay.

 

Dendritic cell-associated C-type lectin-1 (Dectin-1) is a C-type lectin receptor (CLR) that recognizes β-glucans, glucose polymers found in the cell walls of fungi, and thus plays an important role in antifungal innate immunity. Dectin-1  is encoded by the C-type lectin domain family 7 member A (CLEC7A) gene and alternatively spliced into 2 major isoforms: a full-length A isoform and a ‘stalkless’ B isoform, which do not induce the same response to soluble and particulate β-glucans [1, 2].

More details More details

 

The hybridoma-derived Anti-hDectin-1-IgG (mabg-hdect-2) antibody has been replaced by Anti-hDectin-1-mIgG1 (mab9-hdect1), which is produced by recombinant technology and purified from CHO cells.

 

 

Read our review Read our review on Dectin-1 & β-glucans.

Read our review Read our review on Dectin-1 & inflammasomes.

 

References:

1. Brown G.D. et al., 2003. Dectin-1 mediates the biological effects of beta-glucans. J Exp Med. 197: 1119- 24.
2. Heinsbroek S.E. et al., 2006. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 2017;13(6):536-543.

All products are for research use only, and not for human or veterinary use.

Neutralizing and detection antibody against hDectin-1
Neutralizing and detection antibody against hDectin-1

SPECIFICATIONS

Specifications

Target

Dectin-1, CLEC7A

Target species

Human

Applications

Blocking assays, detection assay

Antibody applications
Neutralization assay
Flow cytometry
Species
Human
Isotype
mIgG1
kappa
Recommended isotype control
Recombinant Mouse IgG1 Isotype Control Antibody
Clone
22H8
Tag
Tag-free
Source
CHO cells
Purification
Protein G
Formulation buffer

Sodium phosphate buffer, saccharose, glycine, stabilizing agents

Preservative
Azide-free
Appearance (form)
Lyophilized
Reconstitution buffer
Sterile water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Neutralization assay, flow cytometry

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-hDectin-1-mIgG1
  • Cat code: 
    mab9-hdect1
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Dectin-1 is a member of the C-type lectin receptor (CLR) family and plays an important role in antifungal innate immunity. In humans and mice, it is alternatively spliced into two major isoforms, a full-length A isoform and a ‘stalkless’ B isoform [1, 2]. It is expressed on phagocytic cells, including macrophages and neutrophils.
Dectin-1 is a specific receptor for β-glucans [2]. β-Glucans are glucose polymers found in the cell wall of fungi, including the yeasts Saccharomyces cerevisiae and Candida albicans. Upon binding to its ligand, Dectin-1 triggers phagocytosis and signaling through the kinase Syk and the adaptors CARD9-Bcl10-Malt1 leading to the production of reactive oxygen species (ROS), activation of NF-κB and subsequent production of pro-inflammatory cytokines [3].
Notably, Dectin-1 signaling has been shown to collaborate with Toll‑like receptor 2 (TLR2) signaling to enhance the responses triggered by each receptor [4].

 

1. Fischer M. et al., 2017. Isoform localization of Dectin-1 regulates the signaling quality of anti-fungal immunity. Eur J Immunol. 47(5):848-859.
2. Hou H. et al., 2017. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 13(6):536-543.
3. Drummond RA. & Lionakis MS., 2016. Mechanistic Insights into the Role of C-Type Lectin Receptor/ CARD9 Signaling in Human Antifungal Immunity. Front Cell Infect Microbiol. 6:39.
4. Romero MM. et al., 2016. Reactive oxygen species production by human dendritic cells involves TLR2 and dectin-1 and is essential for efficient immune response against Mycobacteria. Cell Microbiol. 18(6):875-86.

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