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Anti-mouse Dectin-1 (CLEC7A) antibody

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Anti-mDectin-1-IgG

Antibody against murine Dectin-1 (clone R1-8g7)

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2 x 100 µg

mabg-mdect-2
+-
$385
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Mouse Dectin-1 neutralizing and detection antibody - Monoclonal Rat IgG2a

Neutralizing and detection antibody against mDectin-1
Neutralizing and detection antibody against mDectin-1

Anti-mDectin-1-IgG (clone R1-8g7) is a monoclonal rat IgG2a antibody against murine Dectin-1 (mDectin-1). This antibody was screened for neutralization activity and flow cytometry. It is produced in hybridomas and purified by affinity chromatography with protein G.  Anti-mDectin-1-IgG recognizes mDectin-1 isoforms A and B. 

Dendritic cell-associated C-type lectin-1 (Dectin-1) is a C-type lectin receptor (CLR) that recognizes β-glucans, glucose polymers found in the cell walls of fungi, and thus plays an important role in antifungal innate immunity. Dectin-1 is encoded by the C-type lectin domain family 7 member A (CLEC7A) gene and alternatively spliced into 2 major isoforms: a full-length A isoform and a ‘stalkless’ B isoform, which do not induce the same response to soluble and particulate β-glucans [1, 2].

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Key features:

  • Targets murine Dectin-1
  • Provided azide-free
  • Each lot is functionally tested

Application

  • Neutralization assay
  • Flow cytometry

 

Read our review Read our review on Dectin-1 & β-glucans.

Read our review Read our review on Dectin-1 & inflammasomes.

 

References:

1. Brown G.D. et al., 2003. Dectin-1 mediates the biological effects of beta-glucans. J Exp Med. 197: 1119- 24.
2. Heinsbroek S.E. et al., 2006. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 2017;13(6):536-543.

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Specifications

Target: Murine Dectin-1 (mDectin-1)

Specificity: No cross-reactivity with human Dectin-1

Clonality: Monoclonal antibody

Clone: R1-8g7

Isotype: Rat IgG2a, kappa

Control: Rat Control IgG2a

Source: Hybridoma cells

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with saccharose, glycine, and stabilizing agents.

Purity: Purified by affinity chromatography with protein G

Applications: Neutralization/block; Flow cytometry; Immunohistochemistry

Quality control:

  • This product has been validated for neutralization using cellular assays.
  • Binding of Anti-mDectin-1-IgG to mDectin-1 on cells has been validated using flow cytometry.
  • The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
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Contents

  • 2 x 100 µg of purified anti-mDectin-1-IgG, provided azide-free and lyophilized

room temperature Product is shipped at room temperature.

store Upon receipt, store lyophilized antibody at -20°C.

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Details

Dectin-1 is a member of the C-type lectin receptor (CLR) family and plays an important role in antifungal innate immunity. In humans and mice, it is alternatively spliced into two major isoforms, a full-length A isoform and a ‘stalkless’ B isoform [1, 2]. It is expressed on phagocytic cells, including macrophages and neutrophils.
Dectin-1 is a specific receptor for β-glucans [2]. β-Glucans are glucose polymers found in the cell wall of fungi, including the yeasts Saccharomyces cerevisiae and Candida albicans. Upon binding to its ligand, Dectin-1 triggers phagocytosis and signaling through the kinase Syk and the adaptors CARD9-Bcl10-Malt1 leading to the production of reactive oxygen species (ROS), activation of NF-κB and subsequent production of pro-inflammatory cytokines [3].
Notably, Dectin-1 signaling has been shown to collaborate with Toll‑like receptor 2 (TLR2) signaling to enhance the responses triggered by each receptor [4].

 

1. Fischer M. et al., 2017. Isoform localization of Dectin-1 regulates the signaling quality of anti-fungal immunity. Eur J Immunol. 47(5):848-859.
2. Hou H. et al., 2017. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 13(6):536-543.
3. Drummond RA. & Lionakis MS., 2016. Mechanistic Insights into the Role of C-Type Lectin Receptor/ CARD9 Signaling in Human Antifungal Immunity. Front Cell Infect Microbiol. 6:39.
4. Romero MM. et al., 2016. Reactive oxygen species production by human dendritic cells involves TLR2 and dectin-1 and is essential for efficient immune response against Mycobacteria. Cell Microbiol. 18(6):875-86.

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