RAW-Blue™ Cells
RAW-Blue™ cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
NF-κB-SEAP Reporter Cell Line |
3-7 x 10e6 cells |
raw-sp |
You may also need : QUANTI-Blue™ | View more associated products ▼
Mouse Macrophage Reporter Cell Line
RAW-Blue™ cells are derived from the murine RAW 264.7 macrophages with chromosomal integration of a secreted embryonic alkaline phosphatase (SEAP) reporter construct inducible by NF-κB and AP-1. RAW-Blue™ cells express many pattern-recognition receptors (PRRs), including toll-like receptors (TLRs), NOD-like receptors (NLRs), RIG-I-like receptors (RLRs) and C-type lectin receptors (CLRs).
Therefore, RAW-Blue™ cells can be used as:
- TLR reporter cells: they express all TLRs, with the exception of TLR5, at different levels
- NOD reporter cells: they express NOD2 and, to a lesser extent, NOD1
- RLR reporter cells: they express RIG-I and MDA-5
- CLR reporter cells: they express Dectin-1
The presence of agonists of these PRRs induces signaling pathways leading to the activation of NF-κB and AP-1, and the subsequent production of SEAP.
Levels of SEAP can be easily monitored using the detection medium QUANTI-Blue™.
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Specifications
Antibiotic resistance: Zeocin®
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™ supplemented with Zeocin® selective antibiotic only
Guaranteed mycoplasma-free
These cells are covered by a Limited Use License (See Terms and Conditions).
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- 1 vial containing 3-7 x 106 cells
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
Details
Expression of TLR, RLR and NOD mRNAs in RAW-Blue™ cells determined by RT-PCR. RT-PCRs were performed using InvivoGen’s Mouse TLR RT-PCR Primer Set, NOD and RLR RT-Primer Pairs.
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