Invivogen
Menu

RAW-Lucia™ ISG Cells

RAW-Lucia™ ISG Cells Unit size Cat. code Docs Qty Price
Mouse Macrophages - CDS Reporter Cells
3-7 x 10e6 cells
rawl-isg
+-
$1,035.00

Mouse Macrophages - CDS Reporter Cells

RAW-Lucia™ ISG cells were generated from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible Lucia luciferase reporter construct.
RAW 264.7 have been reported to express IFI16 [1], DDX41 and AIM2 [2].

RAW-Lucia™ ISG cells express the Lucia luciferase gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. Thus, RAW-Lucia™ ISG cells allow the monitoring of IRF activation by determining the activity of Lucia luciferase.
The levels of IRF-induced Lucia luciferase in the cell culture supernatant can be easily monitored using QUANTI-Luc™, a luciferase detection reagent. RAW-Lucia™ ISG cells are resistant to Zeocin™.

RAW-Lucia™ ISG cells are responsive to murine IFN-α and IFN-β but do not respond to their human counterparts. They are also responsive to PRR ligands that trigger the IFN signaling pathway, including CDS ligands, such as transfected double stranded DNA, RIG-I ligands, such as transfected double stranded RNA, and STING ligands, such as cGAMP.

 

1. Kawasaki T. et al., 2011. Recognition of nucleic acids by pattern-recognition receptors and its relevance in autoimmunity. Immunol Rev. 243(1):61-73.
2. Stein SC & Falck-Pedersen E., 2012. Sensing adenovirus infection: activation of interferon regulatory factor 3 in RAW 264.7 cells. J Virol. 86(8):4527-37.


Cells were transfected with CDS ligands (1 µg/ml of HSV-60, HSV-60c, VACV-70, VACV-70c, ISD, ISD Control or 0.3 µg/ml pCpGfree-giant, poly(dA:dT), poly(dA), poly(dG:dC) using LyoVec™ or directly stimulated with 10 µg/ml c-di-AMP or c-di-GMP.
After 24h incubation, the levels of IRF-induced Lucia luciferase were determined using QUANTI-Luc™.

Back to the top

Specifications

Antibiotic resistance: Zeocin™

Growth medium: DMEM medium, 2 mM L-glutamine, 10% FBS supplemented with 100 µg/ml Normocin™ and 200 µg/ml Zeocin™.

Guaranteed mycoplasma-free

These products are covered by a Limited Use License (See Terms and Conditions).

Back to the top

Contents

Shipped on dry ice

Back to the top

Citations

Customer Service
& Technical Support

CAPTCHA
In order to prevent automated spam submissions, please answer the following question:
1 + 12 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Shopping cart is empty