Mouse TBK1-KO Reporter RAW 264.7 Cells

TBK1 Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia™ ISG-KO-TBK1 cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the TBK1 gene. TBK1  (TANK-Binding Kinase 1) functions as a key node protein in several cell signaling pathways, including innate immune response, cell growth and proliferation.

RAW-Lucia™ ISG-KO-TBK1 and RAW-Lucia™ ISG cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

RAW-Lucia™ ISG-KO-TBK1 and RAW-Lucia™ ISG cells can be used to study the role of TBK1 by monitoring IRF-induced Lucia luciferase activity. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.

The response of RAW‑Lucia™ ISG-KO-TBK1 cells to murine type I interferons (IFNs) is unaffected by the knockout of the TBK-1 gene. As expected, RAW‑Lucia™ ISG-KO-TBK1 cells do not respond to cyclic dinucleotides (e.g. 2'3'-cGAMP and c-di-AMP) and to transfected double-stranded DNA, such as poly(dA:dT)/LyoVec and VACV70/LyoVec.

RAW-Lucia™ ISG-KO-TBK1 cells are resistant to Zeocin®.