cGAS KO Reporter RAW 264.7 Cells

Product Unit size Cat. code Docs. Qty. Price

RAW-Lucia™ ISG-KO-cGAS Cells

Murine RAW 264.7 macrophages - cGAS Knockout IRF-reporter cells

Show product

3-7 x 10e6 cells


Notification:  This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.

cGAS Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia™ ISG-KO-cGAS cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the cGAS gene.
RAW-Lucia™ ISG-KO-cGAS cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

RAW 264.7 have been reported to express several CDSs, including cGAS [1]. RAW-Lucia™ ISG-KO-cGAS cells allow the monitoring of IRF activation by determining the activity of Lucia luciferase. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.

RAW-Lucia™ ISG-KO-cGAS cells respond to interferons (e.g. IFN-α and IFN-β), cyclic dinucelotides (e.g. 2'3'-cGAMP and c-di-GMP) and transfected poly(I:C). However, as expected, they respond very poorly to transfected DNA, such as poly(dA:dT)/LyoVec and VACV70/LyoVec.

RAW-Lucia™ ISG-KO-cGAS cells are resistant to Zeocin®.



1. Lam E. et al., 2014. Adenovirus Detection by the cGAS/STING/TBK1 DNA Sensing Cascade. J Virol. 88(2):974-81.


Validation of cGAS knockout by PCR
Validation of cGAS knockout by PCR

PCR amplification of the targeted region in the RAW-Lucia™ ISG-KO-cGAS (KO) and RAW-Lucia™ ISG (WT) cells. MWM = molecular weight marker

Validation of cGAS knockout by Western blot (Wes™)
Validation of cGAS knockout by Western blot (Wes™)

Analysis of lysates from the RAW-Lucia™ (WT) and RAW-Lucia™ ISG-KO-cGAS (KO) cells using Anti-cGAS, followed by an HRP-conjugated anti-rabbit secondary antibody. The arrow indicates the expected band for the cGAS protein (59 KDa).

IRF induction in RAW-Lucia™ ISG-KO-cGAS
IRF induction in RAW-Lucia™ ISG-KO-cGAS

Stimulation of RAW-Lucia™ ISG-KO-cGAS and RAW-Lucia™ ISG cells (parental cell line) with poly(dA:dT)/LyoVec™ (1 µg/ml), VACV70/LyoVec™ (1 µg/ml), poly(I:C)/LyoVec™ (1 µg/ml), and 2'3'-cGAMP (3 µg/ml). Mouse IFN-α (1x104 U/ml) and IFN-β (1x104 U/ml) serve as positive controls. Non-induced cells (NI) have been included as a negative control. After a 24h incubation, IRFactivation was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI-Luc™, a Lucia luciferase detection reagent. The IRF induction of each ligand is expressed relative to that of mIFN-β at 1x104 U/ml (taken as 100%).

Back to the top


Antibiotic resistance: Zeocin®

Growth Medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™ supplemented with Zeocin™

Quality Control: 

cGAS knockout is verified by functional assays and DNA sequencing to confirm frameshift mutation/deletion.

The cells are guaranteed mycoplasma-free.

This product is covered by a Limited Use License (See Terms and Conditions).

Back to the top


Dry Ice Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

Back to the top
Customer Service
& Technical Support
Shopping cart is empty