Invivogen
Menu

RAW-Lucia™ ISG-KO-cGAS Cells

RAW-Lucia™ ISG-KO-cGAS Cells Unit size Cat. code Docs Qty Price
Murine RAW 264.7 macrophages - cGAS Knockout IRF-reporter cells
3-7 x 10e6 cells
rawl-kocgas
+-
$1,244.00

cGAS Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia™ ISG-KO-cGAS cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the cGAS gene.
RAW-Lucia™ ISG-KO-cGAS cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

RAW 264.7 have been reported to express several CDSs, including cGAS [1]. RAW-Lucia™ ISG-KO-cGAS cells allow the monitoring of IRF activation by determining the activity of Lucia luciferase. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™, a Lucia luciferase detection reagent.

RAW-Lucia™ ISG-KO-cGAS cells respond to interferons (e.g. IFN-α and IFN-β), cyclic dinucelotides (e.g. 2'3'-cGAMP and c-di-GMP) and transfected poly(I:C). However, as expected, they respond very poorly to transfected DNA, such as poly(dA:dT)/LyoVec and VACV70/LyoVec.

RAW-Lucia™ ISG-KO-cGAS cells are resistant to Zeocin™.

 

1. Lam E. et al., 2014. Adenovirus Detection by the cGAS/STING/TBK1 DNA Sensing Cascade. J Virol. 88(2):974-81.


Stimulation of RAW-Lucia™ ISG-KO-cGAS and RAW-Lucia™ ISG cells (parental cell line) with poly(dA:dT)/LyoVec™ (1 µg/ml), VACV70/LyoVec™ (1 µg/ml), poly(I:C)/LyoVec™ (1 µg/ml), and 2'3'-cGAMP (3 µg/ml). Mouse IFN-α (1x104 U/ml) and IFN-β (1x104 U/ml) serve as positive controls. Non-induced cells (NI) have been included as a negative control. After a 24h incubation, IRFactivation was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI-Luc™, a Lucia luciferase detection reagent. The IRF induction of each ligand is expressed relative to that of mIFN-β at 1x104 U/ml (taken as 100%).

Back to the top

Specifications

Antibiotic resistance: Zeocin™

Growth Medium: DMEM, 4.5 g/l glucose, 10% fetal bovine serum (FBS), 100 µg/ml Normocin™, 2 mM L-glutamine

Quality Control: 

cGAS knockout is verified by functional assays and DNA sequencing to confirm frameshift mutation/deletion. 

The cells are guaranteed mycoplasma-free.

This product is covered by a Limited Use License (See Terms and Conditions).

Back to the top

Contents

Shipped on dry ice (Europe, USA & Canada)

Back to the top

Customer Service
& Technical Support

CAPTCHA
In order to prevent automated spam submissions, please answer the following question:
9 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Shopping cart is empty