Mouse IRF3-KO Reporter RAW 264.7 Cells

IRF-Lucia reporter mouse macrophages

ABOUT

IRF3 knockout reporter mouse macrophages

RAW-Lucia™ ISG-KO-IRF3 cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the IRF3 gene. RAW-Lucia™ ISG-KO-IRF3 cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

IRF-3 is a critical player in the induction of type I IFNs following virus infection. IRF3 is activated by phosphorylation events mediated by the IKK-related kinases TBK-1 and IKKϵ downstream of nucleic acid sensors that detect dsDNA (e.g. cGAS), dsRNA (e.g. RIG-I) or cyclic dinucleotides (STING).

RAW-Lucia™ ISG-KO-IRF3 cells allow the monitoring of IRF activation by determining the activity of Lucia luciferase. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.

RAW-Lucia ISG-KO-IRF3 cells respond to murine type I IFNs but do not respond to cytosolic nucleic acids, such as poly(dA:dT)/LyoVec ,VACV70/LyoVec, poly(I:C)/LyoVec and 2'3'-cGAMP.

RAW-Lucia™ ISG-KO-IRF3 cells are resistant to Zeocin®.

Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

SPECIFICATIONS

Specifications

Species
Mouse
Tested applications

Lucia luciferase activity assay, Functional assays to verify IRF3 knockout

Cell type
Macrophage
Growth properties
Adherent
Tissue origin
Mouse macrophages
Reporter gene
Lucia®
Antibiotic resistance
Zeocin®
Growth medium

Complete DMEM (see TDS)

Mycoplasma-free

Verified using Plasmotest

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    RAW-Lucia™ ISG-KO-IRF3 Cells
  • Cat code: 
    rawl-koirf3
  • Quantity: 
    3-7 x 10^6 cells
Includes:
  • 1 ml of Zeocin® (100 mg/ml)
  • 1 ml of Normocin™ (50 mg/ml)
  • 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)

Shipping & Storage

  • Shipping method:  Dry ice
  • Storage:

    • Liquid nitrogen vapor
    Stability: 20 passages

DOCUMENTS

Documents

RAW-Lucia™ ISG-KO-IRF3 Cells

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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