RAW-Lucia™ ISG-KO-IFI16 Cells
RAW-Lucia™ ISG-KO-IFI16 Cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Murine RAW 264.7 macrophages - IFI16 Knockout IRF-reporter cells |
3-7 x 10e6 cells |
rawl-koif16 |
You may also need : Normocin™ - Antimicrobial Reagent | View more associated products ▼
IFI16 Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages
RAW-Lucia™ ISG-KO-IFI16 cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of murine ortholog of IFI16, p204 (also known as IFI204, PYHIN2).
RAW-Lucia™ ISG-KO-IFI16 cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
RAW 264.7 have been reported to express several CDSs, including IFI16 [1]. RAW-Lucia™ ISG-KO-IFI16 cells can be used to study the importance of IFI16 in the induction of type I IFNs in response to nucleic acids. They allow the monitoring of IRF activation by determining the activity of Lucia luciferase. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™, a Lucia luciferase detection reagent.
RAW-Lucia™ ISG-KO-IFI16 cells are resistant to Zeocin®.
Reference:
1. Stein SC. & Falck-Pedersen E., 2012. Sensing adenovirus infection: activation of interferon regulatory factor 3 in RAW 264.7 cells. J Virol. 86(8):4527-37.
Back to the topSpecifications
Antibiotic resistance: Zeocin®
Growth Medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™ supplemented with Zeocin®
Quality Control:
IFI16/p204 knockout is verified by functional assays and DNA sequencing to confirm frameshift mutation/deletion.
The cells are guaranteed mycoplasma-free.
This product is covered by a Limited Use License (See Terms and Conditions).
Back to the topContents
- 1 vial containing 3-7 x 106 cells
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 pouch of QUANTI-Luc™ (Luciferase detection medium)
Shipped on dry ice (Europe, USA & Canada)