QUANTI-Luc™: Luciferase Detection Reagent
| QUANTI-Luc™ | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Coelenterazine-based reagent for luciferase Flash detection (powder) |
2 pouches (2 x 25ml) 5 pouches (5 x 25ml) |
rep-qlc1 |
| QUANTI-Luc™ Gold | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Coelenterazine-based reagent for luciferase Flash and Glow detection (powder) |
1 pouch (25 ml) 2 pouches (2 x 25 ml) 5 pouches (5 x 25 ml) 1 pouch (500 ml) |
rep-qlcg1 |
| QUANTI-Luc™ 4 Lucia/Gaussia | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Coelenterazine-based reagent for luciferase Flash and Glow detection (liquid) |
500 tests 2 x 500 tests 5 x 500 tests |
rep-qlc4lg1 |
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Notification: QUANTI-Luc™ (powder) and QUANTI-Luc™ Gold (powder) will be discontinued in June 2023 and will be replaced by the liquid formulation: QUANTI-Luc™ 4 Lucia/Gaussia.
Coelenterazine-utilizing luciferase detection reagent
QUANTI-Luc™ is a ready-to-use bioluminescent assay reagent to measure the activity of Lucia luciferase and other coelenterazine-utilizing luciferases, specifically the secreted Gaussia and intracellular Renilla. In order to meet your practical needs, InvivoGen has developed different QUANTI-Luc™ formulations:
– QUANTI-Luc™: powder formulation for Flash detection
– QUANTI-Luc™ Gold: powder formulation for Flash or Glow detection
– QUANTI-Luc™ 4 Lucia/Gaussia: liquid formulation for Flash or Glow detection of Lucia and Gaussia luciferases — NEW !
QUANTI-Luc™, QUANTI-Luc™ Gold, and QUANTI-Luc™ 4 Lucia/Gaussia contain the coelenterazine substrate and stabilizing agents for the luciferase reaction. The light signal produced correlates to the amount of luciferase protein expressed. It is quantified using a luminometer and expressed as relative light units (RLUs). The luminescence can be detected as a "flash" or a "glow" signal. The type of detection depends on the processing workflow:
► Flash luminescence emits light with high intensity for a few seconds. It is ideal for end-point detection in small sample numbers, or when using microplate readers with reagent injectors.
► Glow luminescence emits light with stable intensity for over 10 minutes. It is ideal for high-throughput screening (HTS) (i.e. the typical duration to read a 1536-well plate is ~7 minutes) or when using a luminometer without injectors.
QUANTI-Luc™
- All-in-one powder: QUANTI-Luc™ (coelenterazine substrate + working buffer).
- One-step reagent: just add water to prepare the working solution.
- Flash detection
QUANTI-Luc™ Gold
- Two-component kit: QUANTI-Luc™ Plus (powder) and QLC stabilizer (liquid)
- Flash detection: without stabilizer. This option is ideal for monitoring the cellular response to weak agonists.
- Glow detection: with stabilizer.
|
QUANTI-Luc™ 4 Lucia/Gaussia — NEW !
|
- Two-component kit: QUANTI-Luc™ 4 Reagent (liquid) and QUANTI-Luc™ 4 Stabilizer (liquid),
-
Flash detection: without stabilizer. This option is ideal for monitoring the cellular response to weak agonists.
Equally effective than QUANTI-Luc™ Gold, and QUANTI-Luc™ (see figures). -
Glow detection: with stabilizer.
Equally effective than QUANTI-Luc™ Gold (see figures). - Secreted luciferase detection: assess the activity of Lucia and Gaussia secreted luciferases.
- Convenient liquid formulation: allows extemporaneous dilution; no need for storing a working solution.
➔ QUANTI-Luc™ 4 Lucia/Gaussia has the same detection profile as competitors' reagents
(see Figures and data not shown).
Each lot is thoroughly tested using InvivoGen's Lucia reporter cell lines.
Light signal intensity (Flash detection)
Light signal stability (Glow detection)
Flash detection of secreted Gaussia
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Specifications
QUANTI-Luc™
Cost-effective: One unit is sufficient for 5 x 96 well plates.
Practical: Reconstituted QUANTI-Luc™ is stable for 1 week at 4°C or 1 month at -20°C.
Quality control: Each lot is thoroughly tested in functional assays using the recombinant Lucia protein or Lucia reporter cells.
QUANTI-Luc™ Gold
Cost-effective: One unit is sufficient for 5 x 96-well plates or 2 x 1536-well plates.
Practical: Reconstituted QUANTI-Luc™ Gold is stable for 1 week at 4°C or 1 month at -20°C.
Quality control: Each lot is thoroughly tested in functional assays using the recombinant Lucia protein or Lucia reporter cells.
QUANTI-LUC™ 4 Lucia/Gaussia
Cost-effective: One unit is sufficient for 5 x 96-well plates or 2 x 1536-well plates.
Practical: After preparation, QUANTI-Luc™ 4 Lucia/Gaussia is stable for 48 hours at 4°C and 1 month at -20°C.
Quality control: Each lot is thoroughly tested in functional assays using the recombinant Lucia protein or Lucia reporter cells. The detection of constitutive or inducible Gaussia has been verified using cellular assays.
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QUANTI-Luc™
QUANTI-Luc™ is provided as packs of individually sealed pouches and is available in two pack sizes:
- rep-qlc1: 2 pouches of QUANTI-Luc™
- rep-qlc2: 5 pouches of QUANTI-Luc™
Each pouch contains everything needed to prepare 25 ml of reagent allowing the preparation of 500 wells of a 96-well plate.
QUANTI-Luc™ is shipped at room temperature.
Store at -20°C for up to 12 months.
After preparation, the product is stable for 1 week at 4°C and at least 1 month at -20°C.
Note: This product is photosensitive and should be protected from light.
QUANTI-Luc™ Gold
QUANTI-Luc™ Gold is available in four pack sizes:
- rep-qlcg1: 1 pouch of QUANTI-Luc™ Plus and 1 tube of QLC Stabilizer
- rep-qlcg2: 2 pouches of QUANTI-Luc™ Plus and 2 tubes of QLC Stabilizer
- rep-qlcg 5: 5 pouches of QUANTI-Luc™ Plus and 5 tubes of QLC Stabilizer
One pouch of QUANTI-Luc™ Plus is sufficient for 5 x 96-well plates (standard procedure) or 2 x 1536-well plates (HTS procedure).
- rep-qlcg500: 1 pouch of QUANTI-Luc™ Plus and 1 bottle of QLC Stabilizer
QUANTI-Luc™ Plus (rep-qlcg500) is sufficient for 100 x 96-well plates (standard procedure) or 40 x 1536-well plates (HTS procedure).
QUANTI-Luc™ Plus and QLC Stabilizer are shipped at room temperature.
Upon receipt, store QUANTI-Luc™ Plus at -20°C, and QLC Stabilizer at room temperature (15-25°C).
After preparation, the product is stable for 1 week at 4°C and at least 1 month at -20°C.
Note: This product is photosensitive and should be protected from light.
QUANTI-LUC™ 4 Lucia/Gaussia
QUANTI-Luc™ 4 Lucia/Gaussia is available in three pack sizes:
- rep-qlc4lg1: 1 tube of QUANTI-Luc™ 4 Reagent and 1 tube of QUANTI-Luc™ 4 Stabilizer
- rep-qlc4lg2: 2 tubes of QUANTI-Luc™ 4 Reagent and 2 tubes of QUANTI-Luc™ 4 Stabilizer
- rep-qlc4lg5: 5 tubes of QUANTI-Luc™ 4 Reagent and 5 tubes of QUANTI-Luc™ 4 Stabilizer
One tube of QUANTI-Luc™ 4 Reagent is sufficient for 5 x 96-well plates (standard procedure) or 2 x 1536-well plates (HTS procedure).
Note: This product is photosensitive and should be protected from light.
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