QUANTI-Luc™
| QUANTI-Luc™ | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Coelenterazine-utilizing luciferase detection medium |
2 pouches (2 x 25ml) 5 pouches (5 x 25ml) |
rep-qlc1 |
You may also need : QUANTI-Luc™ Gold | View more associated products ▼
Secreted luciferase detection medium
QUANTI-Luc™ is a lyophilized assay reagent containing all the components required to quantitatively measure the activity of Lucia luciferase and other coelenterazine-utilizing luciferases.
QUANTI-Luc™ is optimized for use with Lucia luciferase reporter cell lines for fast and efficient real-time measurements directly from the cell culture media.
QUANTI-Luc™ contains the coelenterazine substrate for the luciferase reaction, which produces a light signal that is quantified using a luminometer and expressed as relative light units (RLU).
The signal produced correlates to the amount of luciferase protein expressed, indicating promoter activity in the reporter assay.
- One step reagent: just add water to prepare the assay reagent.
- Substrate stability: reconstituted substrate is stable for up to a month, allowing multiple application use.
For increased signal intensity and stability, InvivoGen has developed QUANTI-Luc™ Gold. QUANTI-Luc™ Gold has been optimized for high throughput screening (HTS) assays.
Back to the topSpecifications
Ready to use: Just add water
Cost effective: One pouch is sufficient for 5 x 96 well plates
Practical: Working reagent is stable for at least one month
Use with Lucia luciferase:
- Exceptional sensitivity and reproducibility
- No cell lysis required
- Rapid acquisition of results
Contents
QUANTI-Luc™ is provided as packs of individually sealed pouches.
- rep-qlc1: 2 pouches of QUANTI-Luc™
- rep-qlc2: 5 pouches of QUANTI-Luc™
Each pouch contains everything needed to prepare 25 ml of reagent allowing the preparation of 500 wells of a 96-well plate.
QUANTI-Luc™ is shipped at room temparature.
Store at -20°C up to 12 months.
After preparation, product is stable 1 week at 4°C and at least 1 month at -20°C.
Note: This product is photosensitive and should be protected from light.
Back to the topDetails
QUANTI-Luc™ Procedure
1. Prepare QUANTI-Luc™ reagent by adding 25 ml water to the contents of one pouch.
2. Transfer samples, for instance 10 µl of media from cells containing the secreted luciferase to opaque 96-microwell plates suitable for your luminometer.
3. Set up the luminometer prior to addition of 50 µl QUANTI-Luc™ reagent to each well either manually or by automated injection.
Measure luminosity in endpoint mode when using Lucia luciferase or in kinetic mode depending on the coelenterazine-luciferase used.
