Cloning plasmids with constant regions of the heavy (CH) chain and a secreted luciferase gene
pFUSE-Lucia-CHIg expresses the constant region of the heavy chain (CH) of human, mouse, or rabbit immunoglobulin isotypes. They contain a multiple cloning site (MCS) upstream of the constant region to enable the cloning of the variable (VH) region of a given antibody, Fab or scFv.
Moreover, pFUSE-Lucia-CHIg plasmids contain the Lucia® luciferase gene upstream of the MCS and the CH region to serve as a tag to facilitate the detection and quantification of recombinant antibodies (direct immunofluorescence). Lucia® is a secreted luciferase reporter protein with high protein carrier ability.
As heavy chains cannot be properly secreted in the absence of light chains, the detection of Lucia® luciferase in the cell supernatants reflects the presence of full-length antibodies.