pFUSE-CLIg and pFUSE-CHIg
Isotype switch to generate antibodies with different effector functions (IgA, IgD, IgE, IgG and IgM classes).
Generation of entire antibodies, chimeric, humanized or fully human.
pFUSE-CLIg and pFUSE-CHIg plasmids are designed to change a monoclonal antibody from one isotype to another therefore enabling the generation of antibodies with the same antigen affinity but with different effector functions (increased or reduced ADCC and CDC).
Furthermore, they can be used to produce entire antibodies from fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments that are either chimeric, humanized or fully human depending on the nature of the variable region.