Lucia luciferase Gene
|pSELECT-zeo-Lucia||Unit size||Cat. code||Docs||Qty||Price|
Lucia luciferase Reporter Gene in expression plasmid
InvivoGen provides the Lucia secreted luciferase reporter gene in the pSELECT-zeo plasmid.
It can be used in vivo and in vitro to transfect mammalian cells stably or transiently.
Lucia luciferase gene expression is driven by the EF-1α/HTLV composite promoter that combines the elongation factor 1 alpha core promoter and the 5’untranslated region of the Human T-cell Leukemia Virus.
The pSELECT-zeo-Lucia plasmid contains the zeocin resistance marker for selection in both mammalian cells and bacteria.Back to the top
Selectable with Zeocin™ in both mammalian cells and bacteria.
Provided as lyophilized DNA.
These products are covered by a Limited Use License (See Terms and Conditions).Back to the top
Lucia luciferase reporter gene is provided as 20 µg of lyophilized DNA.
It is supplied with :
- 1 pouch of QUANTI-Luc™
- 2 pouches of E. coli Fast-Media® Zeo TB
- 2 pouches of E. coli Fast-Media® Zeo Agar.
Plasmid is shipped at room temperature.
Store at -20°C for up to one year.Back to the top
High signal sensitivity.
The human embryonic kidney HEK293, the murine melanoma B16 and the murine macrophage RAW 264.7 cell
lines were transiently transfected with 0.1, 0.3 or 1 µg/ml of pSELECT-Lucia-zeo plasmid using the transfection reagent LyoVec™.
Forty-eight hours after transfection, cell supernatants were assessed for luciferase activity using QUANTI-Luc™.
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