pSELECT-zeo-Lucia
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Cat.code:
psetz-lucia
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ABOUT
Lucia® luciferase Reporter Gene in expression plasmid
InvivoGen provides the Lucia® secreted luciferase reporter gene in the pSELECT-zeo plasmid.
It can be used in vivo and in vitro to transfect mammalian cells stably or transiently.
Lucia® luciferase gene expression is driven by the EF-1α/HTLV composite promoter that combines the elongation factor 1 alpha core promoter and the 5’untranslated region of the Human T-cell Leukemia Virus.
The pSELECT-zeo-Lucia plasmid contains the Zeocin® resistance marker for selection in both mammalian cells and bacteria.
Note: Lucia® is a trademark of InvivoGen registered with the United States Patent and Trademark Office and foreign equivalents.
Disclaimer: This product is covered by a Limited Use License (See Terms and Conditions).
Lucia® is a trademark of InvivoGen registered with the United States Patent and Trademark Office and foreign equivalents.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
In vitro transfection
Plasmid construct has been confirmed by restriction analysis and sequencing.
CONTENTS
Contents
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Product:pSELECT-zeo-Lucia
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Cat code:psetz-lucia
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Quantity:20 µg
1 tube of QUANTI-Luc™ 4 Reagent, 1 ml of Zeocin® (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
High signal sensitivity.
The human embryonic kidney HEK293, the murine melanoma B16 and the murine macrophage RAW 264.7 cell lines were transiently transfected with 0.1, 0.3 or 1 µg/ml of pSELECT-Lucia-zeo plasmid using the transfection reagent LyoVec™. Forty-eight hours after transfection, cell supernatants were assessed for luciferase activity using QUANTI-Luc™ 4 Lucia/Gaussia.
DOCUMENTS
Documents
Technical Data Sheet
Plasmid Sequence
Safety Data Sheet
Certificate of analysis
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