InvivoGen offers various human and mouse cell lines to study the STING signaling pathway.
STING (stimulator of interferon genes) is essential in the effective immune response against foreign or self-DNA through the sensing of cytoplasmic cyclic dinucleotides (CDNs), such as 2'3'-cGAMP. The activation of STING causes a TANK-binding-kinase-I (TBK1)-dependent cascade, ultimately, leading to IFN regulatory factor (IRF3)-dependent type I IFN production and NF-κB-dependent inflammatory cytokine production. Additionally, signal transducer and activator of transcription 6 (STAT6) has been reported to be recruited to STING for TBK1-mediated phosphorylation during viral infection.
To facilitate the monitoring of STING-mediated responses, InvivoGen has developed:
– IRF-reporter cell lines
– NF-κB and IRF-reporter cell lines
– STAT6-reporter cell lines
These cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end-user.
Depending on your research needs, you may choose from:
- Human or mouse cell lines
- SEAP (secreted embryonic alkaline phosphatase) and/or Lucia luciferase reporters
- Cell lines deficient for functional STING
- Cells lines expressing a specific STING variant (wild type STING knocked out, followed by a variant STING knocked in)
The activity of the reporter proteins, SEAP and Lucia luciferase, can be conveniently assayed in the cell supernatants using the SEAP detection reagents, QUANTI-Blue™ Solution o HEK-Blue™ Detection, and the Lucia luciferase detection reagent, QUANTI-Luc™ 4 Lucia/Gaussia.
InvivoGen also offers a comprehensive collection of STING products, including STING ligands, a STING inhibitor, a STING antibody, and cloned STING variants into an expression plasmid.
