B16-Blue™ ISG-KO-STING Cells
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Cat.code:
bb-kostg
- Documents
ABOUT
STING Knockout IRF-Inducible SEAP Reporter B16 Melanocytes
B16-Blue™ ISG-KO-STING cells can be used for the study of the STING signaling pathway and for the detection of bioactive murine types I and II IFNs by monitoring the activation of the JAK/STAT pathway.
B16-Blue™ ISG-KO-STING cells were generated from the B16-Blue™ ISG cell line, a murine B16 melanoma-derived cell line, through stable knockout of the STING gene. They express the secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
B16-Blue™ ISG-KO-STING cells do not respond to cytosolic DNA, DMXAA, canonical and non-canonical CDNs while retaining the ability to respond to type I and type II IFNs.
Stimulation of these cells with IFN triggers the activation of the I-ISG54 promoter and the production of SEAP. Levels of SEAP in the supernatant can be easily determined using QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP, by reading the OD at 620-655 nm.
B16-Blue™ ISG-KO-STING cells are resistant to Zeocin®.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
Complete DMEM (see TDS)
Verified using Plasmotest
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:B16-Blue™ ISG-KO-STING Cells
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Cat code:bb-kostg
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Quantity:3-7 x 10^6 cells
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
Storage:
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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