Mouse STING-KO ISG Reporter B16 Cells

IRF-SEAP reporter mouse melanoma -STING deficient

ABOUT

STING Knockout IRF-Inducible SEAP Reporter B16 Melanocytes

B16-Blue™ ISG-KO-STING cells can be used for the study of the STING signaling pathway and for the detection of bioactive murine types I and II IFNs by monitoring the activation of the JAK/STAT pathway.

B16-Blue™ ISG-KO-STING cells were generated from the B16-Blue™ ISG cell line, a murine B16 melanoma-derived cell line, through stable knockout of the STING gene. They express the secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

B16-Blue™ ISG-KO-STING cells do not respond to cytosolic DNA, DMXAA, canonical and non-canonical CDNs while retaining the ability to respond to type I and type II IFNs.

Stimulation of these cells with IFN triggers the activation of the I-ISG54 promoter and the production of SEAP. Levels of SEAP in the supernatant can be easily determined using QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP, by reading the OD at 620-655 nm.

B16-Blue™ ISG-KO-STING cells are resistant to Zeocin®.

Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

SPECIFICATIONS

Specifications

Growth properties
Adherent
Tissue origin
Mouse melanoma
Reporter gene
SEAP
Antibiotic resistance
Zeocin®
Growth medium

Complete DMEM (see TDS)

Mycoplasma-free

Verified using Plasmotest

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    B16-Blue™ ISG-KO-STING Cells
  • Cat code: 
    bb-kostg
  • Quantity: 
    3-7 x 10^6 cells

Shipping & Storage

  • Shipping method:  Dry ice
  • Storage:

    • Liquid nitrogen vapor
    Stability: 20 passages

DOCUMENTS

Documents

B16-Blue™ ISG-KO-STING Cells

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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