B16-Blue™ IFN-α/β Cells

B16-Blue™ IFNα/β cells Unit size Cat. code Docs Qty Price
Murine Type I IFNs Sensor Cells
3-7 x 10e6 cells

You may also need : QUANTI-Blue™ | View more associated products

Please note that, for your convenience, these cells are now provided with QUANTI-Blue™ Solution.

B16-Blue™ IFN-α/β

Murine Type I IFNs Sensor Cells

B16-Blue™ IFN-α/β cells allow the detection of bioactive murine type I IFNs by monitoring the activation of the JAK/STAT/ISGF3 pathway and/or IRF3 pathway.

They derive from the murine B16 melanoma cell line of C57BL/6 origin after stable transfection with a SEAP reporter gene under the control of the IFN-α/β-inducible ISG54 promoter enhanced by a multimeric ISRE.

B16-Blue™ IFN-α/β cells do not respond to IFN-γ, due to the inactivation of IFN-γ receptor. B16-Blue™ IFN-α/β cells respond specifically to mIFN-α/β and do not respond to human IFN-α/β.

Stimulation of B16-Blue™ IFN-α/β cells with murine IFN-α or IFN-β, or type I IFN inducers, such as poly(I:C), poly(dA:dT) or 5’ppp-dsRNA delivered intracelluarly, triggers the production of SEAP by the activation of the IRF-inducible promoter.

Levels of SEAP can be easily monitored using the detection medium QUANTI-Blue™.


RIG-I response to 5'ppp-dsRNA and 5'ppp-dsRNA Control in B16-Blue™ ISG cells
RIG-I response to 5'ppp-dsRNA and 5'ppp-dsRNA Control in B16-Blue™ ISG cells

RIG-I activation with 5’ppp-dsRNA can be easily monitored using B16 Blue™ ISG cells, an IFN regulatory factor (IRF)-inducible secreted embryonic alkaline phosphatase (SEAP) reporter cell line. B16-Blue™ ISG cells respond to stimulation with 5’ppp-dsRNA and Poly(I:C). The 5’ppp-dsRNA Control (non-phosphorylated) does not induce any response in B16-Blue™ ISG cells. Levels of SEAP is determined by colorimetric measurement using QUANTI-Blue™ and reading optical density (O.D.) at 630-655 nm.

Stimulation of B16-Blue™ IFN-α/β cells
Stimulation of B16-Blue™ IFN-α/β cells

Stimulation of B16-Blue™ IFN-α/β cells by recombinant human and murine IFN-α and IFN-β was assessed by measuring the levels of SEAP using QUANTI-Blue™.

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Detects murine type I interferons:

  • Detection range for mouse IFN-α: 102 - 104 IU/ml
  • Detection range for mouse IFN-β: 102 - 104 IU/ml

Antibiotic resistance: Zeocin™

Growth medium: DMEM, 10% (v/v) heat-inactivated fetal bovine serum, 2 mM L-glutamine, 100 µg/ml Normocin™, 100 U/ml penicillin, 100 µg/ml streptomycin

Guaranteed mycoplasma-free


This product is covered by a Limited Use License (See Terms and Conditions).

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  • 1 vial containing 3-7 x 106 cells
  • 1 ml of Zeocin™ (100 mg/ml)
  • 1 ml of Normocin™ (50 mg/ml)
  • 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)

Shipped on dry ice (Europe, USA & Canada)

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Any questions about our cell lines ? Visit our frequently asked questions page

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