B16-Blue™ IFN-α/β Cells
|B16-Blue™ IFNα/β cells||Unit size||Cat. code||Docs||Qty||Price|
Murine Type I IFNs Sensor Cells
B16-Blue™ IFN-α/β cells allow the detection of bioactive murine type I IFNs by monitoring the activation of the JAK/STAT/ISGF3 pathway and/or IRF3 pathway.
They derive from the murine B16 melanoma cell line of C57BL/6 origin after stable transfection with a SEAP reporter gene under the control of the IFN-α/β-inducible ISG54 promoter enhanced by a multimeric ISRE.
B16-Blue™ IFN-α/β cells do not respond to IFN-γ, due to the inactivation of IFN-γ receptor. B16-Blue™ IFN-α/β cells respond specifically to mIFN-α/β and do not respond to human IFN-α/β.
Stimulation of B16-Blue™ IFN-α/β cells with murine IFN-α or IFN-β, or type I IFN inducers, such as poly(I:C), poly(dA:dT) or 5’ppp-dsRNA delivered intracelluarly, triggers the production of SEAP by the activation of the IRF-inducible promoter.
Levels of SEAP can be easily monitored using the detection medium QUANTI-Blue™.
Stimulation of B16-Blue™ IFN-α/β cells by recombinant human and murine IFN-α and IFN-β was assessed by measuring the levels of SEAP using QUANTI-Blue™.
Detects murine type I interferons:
- Detection range for mouse IFN-α: 102 - 104 IU/ml
- Detection range for mouse IFN-β: 102 - 104 IU/ml
Antibiotic resistance: Zeocin™.
Growth medium: RPMI, 2 mM L- glutamine,10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™.
Guaranteed mycoplasma-free.Back to the top
1 vial containing 3-7 x 106 cells
100 μl Zeocin™ (100 mg/ml)
1 ml Normocin™ (50 mg/ml)
1 pouch of QUANTI-Blue™ (SEAP detection medium)
Shipped on dry iceBack to the top
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