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B16-Blue™ IFN-α/β Cells

B16-Blue™ IFNα/β cells Unit size Cat. code Docs Qty Price
Murine Type I IFNs Sensor Cells
3-7 x 10e6 cells
bb-ifnt1
+-
$1,182.00

May, 17th 2019 - CONTENTS UPDATE NOTIFICATION
Please note that, for your convenience, these cells are now provided with QUANTI-Blue™ Solution.

Murine Type I IFNs Sensor Cells

B16-Blue™ IFN-α/β cells allow the detection of bioactive murine type I IFNs by monitoring the activation of the JAK/STAT/ISGF3 pathway and/or IRF3 pathway.

They derive from the murine B16 melanoma cell line of C57BL/6 origin after stable transfection with a SEAP reporter gene under the control of the IFN-α/β-inducible ISG54 promoter enhanced by a multimeric ISRE.

B16-Blue™ IFN-α/β cells do not respond to IFN-γ, due to the inactivation of IFN-γ receptor. B16-Blue™ IFN-α/β cells respond specifically to mIFN-α/β and do not respond to human IFN-α/β.

Stimulation of B16-Blue™ IFN-α/β cells with murine IFN-α or IFN-β, or type I IFN inducers, such as poly(I:C), poly(dA:dT) or 5’ppp-dsRNA delivered intracelluarly, triggers the production of SEAP by the activation of the IRF-inducible promoter.

Levels of SEAP can be easily monitored using the detection medium QUANTI-Blue™.

Figures

Response of B16-Blue™ IFN-α/β cells
Response of B16-Blue™ IFN-α/β cells

Response of B16-Blue™ IFN-α/β cells following their stimulation with poly(I:C)-HMW, poly(dA:dT), 5’ppp-dsRNA, 5’ppp-dsRNA control delivered intracellularly using the transfection reagent, LyoVec™. Induction of type I IFNs was assessed by determining the levels of SEAP using QUANTI-Blue™.

Stimulation of B16-Blue™ IFN-α/β cells
Stimulation of B16-Blue™ IFN-α/β cells

Stimulation of B16-Blue™ IFN-α/β cells by recombinant human and murine IFN-α and IFN-β was assessed by measuring the levels of SEAP using QUANTI-Blue™.

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Specifications

Detects murine type I interferons:

  • Detection range for mouse IFN-α: 102 - 104 IU/ml
  • Detection range for mouse IFN-β: 102 - 104 IU/ml

Antibiotic resistance: Zeocin™

Growth medium: DMEM, 10% (v/v) heat-inactivated fetal bovine serum, 2 mM L-glutamine, 100 µg/ml Normocin™, 100 U/ml penicillin, 100 µg/ml streptomycin

Guaranteed mycoplasma-free

 

This product is covered by a Limited Use License (See Terms and Conditions).

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Contents

  • 1 vial containing 3-7 x 106 cells
  • 1 ml of Zeocin™ (100 mg/ml)
  • 1 ml of Normocin™ (50 mg/ml)
  • 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)

Shipped on dry ice (Europe, USA & Canada)

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Details

B16-blue-IFNanlpha/beta Pathway

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Citations

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