B16-Blue™ IFN-α/β Cells
B16-Blue™ IFN-α/β cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Murine Type I IFNs Sensor Cells |
3-7 x 10e6 cells |
bb-ifnt1 |
You may also need : QUANTI-Blue™ | View more associated products ▼
Notification: This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.
Murine Type I IFNs Sensor Cells
B16-Blue™ IFN-α/β cells signaling
B16-Blue™ IFN-α/β cells allow the detection of bioactive murine type I IFNs (i.e. IFN-α and IFN-β) by monitoring the activation of the JAK/STAT/ISGF3 pathway and/or IRF3 pathway.
IFN-α and IFN-β are important anti-viral cytokines that also have anti-proliferative and immunomodulatory functions [1, 2]. These cytokines bind a cell-surface receptor, composed of two subunits, IFNAR1 and IFNAR2, which are associated with TyK2 and JAK1, respectively [1]. Upon binding to this receptor, type I IFNs trigger the JAK/STAT/ISGF3 pathway.
Cell line description:
B16-Blue™ IFN-α/β cells derive from the murine B16 melanoma cell line of C57BL/6 origin after stable transfection with a SEAP (secreted embryonic alkaline phosphatase) reporter gene under the control of the IFN-α/β-inducible ISG54 promoter enhanced by a multimeric ISRE. These cells do not respond to IFN-γ, due to the inactivation of the IFN-γ receptor.
B16-Blue™ IFN-α/β cells respond specifically to mIFN-α/β and do not respond to human IFN-α/β. Stimulation of these cells with murine IFN-α or IFN-β, or type I IFN inducers, such as poly(I:C), poly(dA:dT) or 5’ppp-dsRNA delivered intracellularly, triggers the production of SEAP by the activation of the IRF-inducible promoter. Levels of SEAP can be easily monitored using the detection medium QUANTI-Blue™ Solution.
Features of B16-Blue™ IFN-α/β cells:
- Fully functional murine IFN-α/β signaling pathway
- Do not respond to human IFN-α/β
- Do not respond to murine IFN-γ (type II IFN)
- Readily assessable SEAP reporter activity
- Functionally tested and guaranteed mycoplasma-free
Applications of B16-Blue™ IFN-α/β cells:
- Detection of murine IFN-α and IFN-β
- Screening of anti-mIFN-α or anti-mIFN-β antibodies
References:
1. Schreiber G. 2017. The molecular basis for differential type I interferon signaling. J. Biol. Chem. 292:7285-94.
2. McNab F. et al., 2015. Type I interferons in infectious disease. Nat Rev Immunol. 15(2):87-103.
Specifications
Detects murine type I interferons:
- Detection range for mouse IFN-α: 102 - 104 IU/ml
- Detection range for mouse IFN-β: 102 - 104 IU/ml
Antibiotic resistance: Zeocin®
Growth medium: DMEM, 10% (v/v) heat-inactivated fetal bovine serum, 2 mM L-glutamine, 100 µg/ml Normocin™, 100 U/ml penicillin, 100 µg/ml streptomycin
Guaranteed mycoplasma-free
This product is covered by a Limited Use License (See Terms and Conditions).
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- 1 vial containing 3-7 x 106 cells
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
Details
Type I interferons, in particular interferon-alpha (IFN-α) and interferon beta (IFN-β), play a vital role in host resistance to viral infections [1, 2]. The type I IFN family is a multi-gene cytokine family that encodes 14 partially homologous IFN-α subtypes in mice (13 in humans), a single IFN-β, and several poorly defined single gene products (IFN-ɛ, IFN-τ, IFN-κ, IFN-ω, IFN-δ, and IFN-ζ) [1, 2]. IFN-α and IFN-β are the best-defined and most broadly expressed type I IFNs [2].
IFN-β and all of the IFN-α subtypes bind to a heterodimeric transmembrane receptor composed of the subunits IFNAR1 and IFNAR2 which are associated with the tyrosine kinases Tyk2 and Jak1 (Janus kinase 1) respectively. These kinases phosphorylate STAT1 and STAT2 which then dimerize and interact with IFN regulatory factor 9 (IRF9), leading to the formation of the ISGF3 complex. ISGF3 binds to IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG) to regulate their expression.
1. Schreiber G. 2017. The molecular basis for differential type I interferon signaling. J. Biol. Chem. 292:7285-94.
2. McNab F. et al., 2015. Type I interferons in infectious disease. Nat Rev Immunol. 15(2):87-103.