|5'ppp-dsRNA||Unit size||Cat. code||Docs||Qty||Price|
5' triphosphate double stranded RNA
5’ triphosphate double stranded RNA (5’ ppp-dsRNA) is a synthetic ligand for the retinoic acid-inducible protein I (RIG-I).
RIG-I is a cytosolic pattern recognition receptor that senses pathogen-associated molecular patterns (PAMPs) on viral RNA and triggers an antiviral immune response by the activation of type-I interferons (IFN-α and -β).Back to the top
Specificity: RIG-I agonist
Working Concentration: 300 ng- 1 μg/ml
Endotoxin level: EndoFit™ (<0.001 EU/µg)
Solubility: 0.1 mg/ml in water
5’- pppGCAUGCGACCUCUGUUUGA -3’ (19 mer)
3’- CGUACGCUGGAGACAAACU -5’ (19 mer)
5’ triphosphate double stranded RNA is obtained by hybridization of one 5’ triphosphate single-stranded 19-mer phosphodiester RNA with its complementary strand (non-triphosphatase).
Each strand is chemically synthesized by solid-phase synthesis and purified by reverse phase HPLC.Back to the top
5’ppp-dsRNA Naked available in two quantities:
- 25 μg 5’ppp-dsRNA naked.
- 2 ml sterile endotoxin-free water.
- 100 μg (4x25μg) 5’ppp-dsRNA naked.
- 10 ml sterile endotoxin-free water.
5’ppp-dsRNA Naked is provided lyophilized and shipped at room temperature.
Store lyophilized 5’ppp-dsRNA at -20°C.
Lyophilized product is stable for 1 year when stored -20°C.Back to the top