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5′ppp-dsRNA

5'ppp-dsRNA Unit size Cat. code Docs Qty Price
5' triphosphate double stranded RNA
25 µg
100 µg
tlrl-3prna
+-
$145.00

You may also need : 3p-hpRNA [ NEW ] | View more associated products

RIG-I agonist

5’ triphosphate double stranded RNA (5’ ppp-dsRNA) is a synthetic ligand for the retinoic acid-inducible protein I (RIG-I).
RIG-I is a cytosolic pattern recognition receptor that senses pathogen-associated molecular patterns (PAMPs) on viral RNA and triggers an antiviral immune response by the activation of type-I interferons (IFN-α and -β).

5’ ppp-dsRNA is also available complexed with the cationic lipid transfection reagent LyoVec™ to facilitate its uptake.

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Specifications

Specificity: RIG-I agonist

Working Concentration: 300 ng- 1 μg/ml

Endotoxin level:  EndoFit™ (<0.001 EU/µg)

Solubility: 0.1 mg/ml in water

Formulation: naked

5’ppp-dsrna:
5’- pppGCAUGCGACCUCUGUUUGA -3’ (19 mer)
      3’- CGUACGCUGGAGACAAACU -5’ (19 mer)

5’ triphosphate double stranded RNA is obtained by hybridization of one 5’ triphosphate single-stranded 19-mer phosphodiester RNA with its complementary strand (non-triphosphatase).

Each strand is chemically synthesized by solid-phase synthesis and purified by reverse phase HPLC.

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Contents

5’ppp-dsRNA Naked available in two quantities:

tlrl-3prna:

  • 25 μg 5’ppp-dsRNA naked.
  • 2 ml sterile apyrogenic water.

tlrl-3prna-100:

  • 100 μg (4x25μg) 5’ppp-dsRNA naked.
  • 10 ml sterile apyrogenic water.

5’ppp-dsRNA Naked is provided lyophilized and shipped at room temperature.

Store lyophilized 5’ppp-dsRNA at -20°C.

Lyophilized product is stable for 1 year when stored -20°C.

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Citations

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