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5′ppp-dsRNA Control

5'ppp-dsRNA Control Unit size Cat. code Docs Qty Price
Control for 5'ppp-dsRNA
25 µg
100 µg
tlrl-3prnac
+-
$102.00

Negative control for 5′ppp-dsRNA

5’ppp-dsRNA Control is a 19 mer blunt-end dsRNA without a 5’triphosphate.

5’ppp-dsRNA Control is a negative control for 5’ triphosphate double stranded RNA (5’ ppp-dsRNA), a synthetic ligand for RIG-I.

5’ ppp-dsRNA is also available complexed with the cationic lipid transfection reagent LyoVec™ to facilitate its uptake.

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Specifications

Working Concentration: 300 ng- 1 μg/ml
Endotoxin level:  EndoFit™ (<0.001 EU/µg)
Solubility: 0.1 mg/ml in water
Formulation: naked

Sequence:
5’- GCAUGCGACCUCUGUUUGA -3’ (19 mer)
3’- CGUACGCUGGAGACAAACU -5’ (19 mer)

The double stranded RNA Control is obtained by hybridization of non-triphosphatase single-stranded 19-mer phosphodiester RNA with its complementary strand (also non-triphosphatase). Each strand is chemically synthesized by solid-phase synthesis and purified by reverse phase HPLC.

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Contents

5’ppp-dsRNA Control Naked is available in two quantities:
- 25 μg 5’ppp-dsRNA Control
- 2ml sterile apyrogenic water
or
- 100 μg (4x25μg) 5’ppp-dsRNA Control
- 10ml sterile apyrogenic water

5’ppp-dsRNA Control / LyoVec is available in two quantities:
- 25 μg 5’ppp-dsRNA Control / LyoVec
or
- 100 μg (4x25μg) 5’ppp-dsRNA Control/ LyoVec

Products are provided lyophilized and shipped at room temperature. Store lyophilized 5’ppp-dsRNA Control at -20 ̊C. Lyophilized product is stable for 1 year when stored -20°C.

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Details

B16-Blue™ IFN α/β cells respond to stimulation with 5'ppp-dsRNA and poly(I:C) when transfected with LyoVec™

B16-Blue™ ISG cells respond to stimulation with 5'ppp-dsRNA and poly(I:C) when transfected with LyoVec™

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Citations

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