|5'ppp-dsRNA Control||Unit size||Cat. code||Docs||Qty||Price|
Control for 5'ppp-dsRNA
|5'ppp-dsRNA Control / LyoVec™||Unit size||Cat. code||Docs||Qty||Price|
Control ligand for RIG-I - complexed with transfection reagent
Negative control for 5′ppp-dsRNA
5’ppp-dsRNA Control is a 19 mer blunt-end dsRNA without a 5’triphosphate.
5’ppp-dsRNA Control is a negative control for 5’ triphosphate double stranded RNA (5’ ppp-dsRNA), a synthetic ligand for RIG-I.
5’ ppp-dsRNA is also available complexed with the cationic lipid transfection reagent LyoVec™ to facilitate its uptake.Back to the top
Working Concentration: 300 ng- 1 μg/ml
Endotoxin level: EndoFit™ (<0.001 EU/µg)
Solubility: 0.1 mg/ml in water
5’- GCAUGCGACCUCUGUUUGA -3’ (19 mer)
3’- CGUACGCUGGAGACAAACU -5’ (19 mer)
The double stranded RNA Control is obtained by hybridization of non-triphosphatase single-stranded 19-mer phosphodiester RNA with its complementary strand (also non-triphosphatase).
Each strand is chemically synthesized by solid-phase synthesis and purified by reverse phase HPLC.Back to the top
5’ppp-dsRNA Control Naked is available in two quantities:
- 25 μg 5’ppp-dsRNA Control
- 2ml sterile apyrogenic water
- 100 μg (4x25μg) 5’ppp-dsRNA Control
- 10ml sterile apyrogenic water
Products are provided lyophilized and shipped at room temperature.
Store lyophilized 5’ppp-dsRNA Control at -20 ̊C.
Lyophilized product is stable for 1 year when stored -20°C.Back to the top
B16-Blue™ ISG cells respond to stimulation with 5'ppp-dsRNA and poly(I:C) when transfected with LyoVec™Back to the top