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Poly(dA:dT)

Poly(dA:dT) naked Unit size Cat. code Docs Qty Price
dsDNA naked
200 µg
1 mg
tlrl-patn
+-
$124.00

Synthetic analog of B-DNA

Poly(dA:dT) is a poly(deoxyadenylic-deoxythymidylic) acid sodium salt. It is a repetitive synthetic double-stranded DNA sequence of poly(dA-dT)•poly(dT-dA) and a synthetic analog of B-DNA. Poly(dA:dT) is recognized by several sensors, including DAI, LRRFIP1 and AIM2. Poly(dA:dT) is also indirectly detected by RIG-I. The activity of poly(dA:dT) is tested using the reporter cell line B16-Blue™ IFNα/β.

Poly(dA:dT) is also available complexed with the cationic lipid transfection reagent LyoVec™ to facilitate its uptake.

 

 

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Specifications

Activity: RIG-I & CDS agonist, AIM2 inflammasome inducer

Formulation: naked

CAS number: 86828-69-5

Solubility: 2 mg/ml in physiological water

Endotoxin level: EndoFit™ (<0.001 EU/µg)

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Contents

Poly(dA:dT) naked is available in two quantities:
- 200 µg lyophilised poly(dA:dT) naked
  2 ml sterile endotoxin-free physiological water (150 mM NaCl)
- 1 mg lyophilised poly(dA:dT) naked
  2 ml sterile endotoxin-free physiological water (150 mM NaCl)

Poly(dA:dT) / LyoVec:
- 4 x 25 μg lyophilized poly(dA:dT)/LyoVec™
Note: Each vial contains 25 μg of poly(dA-dT)•poly(dT-dA) complexed with 50 μg LyoVec.
- 10 ml sterile endotoxin-free water

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Description

Intracellular poly(dA:dT) is recognized by several cytosolic DNA sensors, such as ZBP1/DAI and LRRFIP1, triggering an immune response [1-5]. ZBP1/DAI and LRRFIP1 bind poly(dA:dT) inducing type I IFN production via TBK1/IRF-3 and β-catenin pathways, respectively [3,4]. Additionally, poly(dA:dT) is recognized by AIM2 triggering the formation of an inflammasome and the subsequent secretion of IL-1β and IL-18 [5]. Furthermore, transfected poly(dA:dT) can be transcribed by RNA polymerase III into dsRNA with a 5’-triphosphate moiety (5’ppp-dsRNA) which is a ligand for RIG-I [6,7]. Thus poly(dA:dT) is indirectly sensed by RIG-I leading to type I IFN production through the adaptor molecule IPS-1 and the TBK1/IRF3 pathway [8].

1. Ishii KJ, et al., 2006. A Toll-like receptor-independent antiviral response induced by doublestranded B-form DNA. Nat Immunol. 7(1):40-8.
2. Ishii KJ. & Akira S., 2006. Innate immune recognition of, and regulation by, DNA Trends Immunol. 27(11):525-32.
3. Takaoka A. et al., 2007. DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response. Nature. 448(7152):501-5.
4. Yang P. et al., 2010. The cytosolic nucleic acid sensor LRRFIP1 mediates the production of type I interferon via a beta-catenin-dependent pathway. Nat Immunol. 11(6):487-94.
5. Jones JW. et al., 2010. Absent in melanoma 2 is required for innate immune recognition of Francisella tularensis. PNAS, 107(21):9771-6.
6. Ablasser A. et al., 2009. RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate. Nat Immunol. 10(10):1065-72.
7. Chiu YH. et al., 2009. RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway. Cell. 138(3):576-91.
8. Takeshita F. & Ishii KJ., 2008. Intracellular DNA sensors in immunity. Curr Opin Immunol. 20(4):383-8.

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Details

Responses of B16-Blue IFNalpha/beta cells to dsDNA and dsRNA

Responses of B16-Blue™ IFNα/β cells to dsDNA and dsRNA:

B16-Blue™ IFNα/β cells were stimulated with increasing concentrations of poly(dA:dT), poly(dG:dC) or 5’ppp-dsRNA complexed extemporaneously with the transfection reagent LyoVec™.
After 24h incubation, induction of type I IFNs was assessed by determining the levels of SEAP using QUANTI-Blue™, a SEAP detection medium.

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Citations

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