3p-hpRNA - RIG-I Agonist

5’ triphosphate hairpin RNA

ABOUT

RIG-I agonist

3p-hpRNA is a 5’ triphosphate hairpin RNA that was generated by in vitro transcription of a sequence from the influenza A (H1N1) virus, a single‑stranded negative‑sense RNA virus [1,2]. This 89-mer RNA oligonucleotide contains an uncapped 5’ triphosphate extremity and a double-strand fragment. 3p-hpRNA sequence self-anneals to form secondary structures such as hairpin or panhandle conformations. These structural features, which distinguish viral RNA from mammalian RNA, are recognized by retinoic acid-inducible gene I (RIG‑I) [3,4]. RIG-I is a cytoplasmic RNA helicase that belongs to the RIG-I-like receptors (RLRs) family and triggers an antiviral immune response by the activation of type-I interferons (IFN-α and -β)[5].

To achieve stimulation of RIG-I, 3p-hpRNA must be delivered into the cytoplasm, for example by using a transfection agent, such as LyoVec™. Of note, this ligand induces stronger RIG-I responses than 5’ppp‑dsRNA, a fully synthetic RIG-I ligand.

Key Features of 3p-hpRNA:

  • 3p-hpRNA is a specific RIG-I agonist
  • Stronger RIG-I responses than 5’ppp‑dsRNA
  • Its biological activity has been verified using our RLR reporter cell lines.

 

References:

1. Rehwinkel J. et al., 2010. RIG-I detects viral genomic RNA during negative-strand RNA virus infection. Cell. 140:397-408.
2. Liu G. et al., 2015. Influenza A Virus Panhandle Structure Is Directly Involved in RIG-I Activation and IFN Induction. J Virol. 89(11):6067-79.
3. Hornung V. et al., 2006. 5’-triphosphate RNA is the ligand for RIGI. Science. 314:994-7.
4. Gebhardt A. et al., 2017. Discrimination of Self and Non-Self Ribonucleic Acids. Journal of IFN & Cytokine Research 37: 184-97.
5. Yoneyama M. et al., 2015. Viral RNA detection by RIG-I-like receptors. Curr Opin Immunol. 32:48-53.

 

Read our review Read our review on RIG-I and cancer immunotherapy.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

RIG-I 

Sequence

5’-pppGGAGCAAAAGCAGGGUGACAAAGACAUAAUGGAUCCAAACACUGUGUCAAGCUUUCAGGUAGAUUGCUUUCUUUGGCAUGUCCGCAAAC- 3’ (89 mer)

Working concentration

10 ng -1 μg/ml for cellular assays

Formulation buffer

Naked

Appearance (form)
Lyophilized
Reconstitution buffer
Sterile endotoxin-free water (provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Cellular assays

Quality control

Each lot is functionally tested and validated using A549-Dual™ Cells.

CONTENTS

Contents

  • Product: 
    3p-hpRNA
  • Cat code: 
    tlrl-hprna
  • Quantity: 
    25 µg
Includes:

1.5 ml sterile endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • Upon receipt, store product at -20 °C
    Stability: The reconstituted product is stable up to 3 months at -20 °C.

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

3p-hpRNA

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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