HEK293 embryonic kidney cell lines

Engineered human embryonic kidney (HEK) 293 cells - Reporter and non-reporter cells

InvivoGen provides a collection of HEK293-derived embryonic kidney cell lines. HEK293 cells are immortalized kidney cells isolated from a human embryo and are among the most widely used in basic, pharmaceutical, and medical research. These adherent epithelial-like cells are very popular, in part because of their robust and rapid growth in culture, as well as their propensity for transfection. HEK293 cells have been used extensively in receptor signaling, cancer research, and large-scale protein production. The HEK293 cell line and its derivatives naturally express only a few pattern-recognition receptors (PRRs), including TLR3, TLR5, and NOD1. Thus they offer the great advantage of enabling the study of a specific signaling pathway upon transfection with genes encoding a given PRR. 

All our cell lines are extensively tested for viabilitystabilitybiological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end-user.

HEK293 cell line collection

  • NF-κB/IRF Reporter Cells
  • ALPK1/TIFA Reporter Cells
  • CLR Reporter Cells
  • NOD Reporter Cells
  • RLR Reporter Cells
  • STING Reporter Cells
  • TLR Reporter Cells
  • Cytokine Reporter Cells
  • Tet-on Inducible Cells
  • SARS-CoV-2 Related Cells

Key features

  • Verified overexpression and/or knockout of important PRRs
  • Stable expression of one or two reporter systems 
  • Quantifiable responses upon stimulation with pathogen/damage-associated molecular patterns (PAMPs/DAMPs)
  • Functionally tested and guaranteed mycoplasma-free


InvivoGen's HEK293 Reporter Cells feature one or two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase. As a result, these cells allow the simultaneous study of the NF-κB and/or the IRF pathways, by assessing the activity of SEAP or the secreted Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent. 

The Tet-on inducible HEK-RepTor™ cells stably and constitutively express an optimized tetracycline repressor (TetR) construct. They are designed for the tetracycline-inducible expression of a gene of interest (GOI), including cytotoxic proteins. 

Our SARS-CoV-2-related HEK cells stably overexpress one or two genes encoding for the SARS-CoV-2 receptors, human ACE2, and TMPRSS2. In addition, they may feature the Dual™ SEAP and Lucia reporter system.


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