HEK-Blue™ KD-TLR5 Cells

TLR5 deficient, IL-1β reporter cells

HEK-Blue™ KD-TLR5 cells are designed to monitor bioactive IL-1β secreted by THP-1 cells upon flagellin-induced NLRC4 activation.

HEK-Blue™ KD-TLR5 cells are derived from the HEK293 cell line, which endogenously expresses TLR5 and the IL-1β receptor (IL-1R).

This cell line features an NF-κB-inducible SEAP reporter gene and was engineered to knock down the expression of TLR5 to avoid activation of NF-κB upon flagellin-induced TLR5 stimulation.

The knockdown of TLR5 permits the analysis of flagellin specifically for its NLRC4 stimulating activity.

The binding of IL-1β to IL-1R initiates a signaling cascade leading to the activation of NF-κB and the subsequent production of SEAP. The detection of SEAP in the supernatant of HEK-Blue™ KD-TLR5 cells can be readily assessed using the QUANTI-Blue™ assay.