THP1-NLRC4 Cells
THP1-NLRC4 cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
NLRC4 inflammasome test cell line |
3-7 x 10e6 cells |
thp-nlrc4 |
You may also need : Normocin™ - Antimicrobial Reagent | View more associated products ▼
NLRC4 inflammasome test cell line
THP1-NLRC4 cells are derived from the THP1 human monocytic cell line, which represents the most commonly used model cell line to study inflammasome activation.
THP1-NLRC4 cells stably overexpress NLRC4 and naturally express TLR5. Stimulation of these cells with flagellin triggers TLR5 signaling leading to NF-κB activation and the production of pro-IL1β. Once in the cytosol, flagellin induces the formation of the NLRC4 inflammasome resulting in the activation of caspase-1 and the release of IL-1β.
Levels of IL-1β secreted into the supernatant of THP-1 cells can be monitored using the HEK-Blue™ KD-TLR5 cell line.
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Antibiotic resistance: Blasticidin
Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)
Quality control:
- Expression of NLRC4 was confirmed by qRT-PCR and a functionality assay using inflammasome inducers.
- THP1-NLRC4 cells are guaranteed mycoplasma-free.
Contents
- 1 vial of THP1-NLRC4 cells (3-7 x 10e6 cells)
- 1 ml of Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.
- 1 ml of Blasticidin (10 mg/ml).
Shipped on dry ice (Europe, USA & Canada)
Description
Application
THP1-NLRC4 cells are designed to study the activation of the NLRC4 inflammasome.
Mature IL-1β can be detected by Western blot, ELISA, or a cell-based assay.
InvivoGen has developed HEK-Blue™ KD-TLR5 cells, a reporter cell line designed to monitor bioactive IL-1β secreted by THP-1 cells upon flagellin-induced NLRC4 activation.
HEK-Blue™ KD-TLR5 cells are derived from the HEK293 cell line, which endogenously expresses TLR5 and the IL-1β receptor (IL-1R). This cell line features an NF-kB-inducible SEAP reporter gene and was engineered to knock-down the expression of TLR5 to avoid activation of NF-kB upon flagellin-induced TLR5 stimulation. The knockdown of TLR5 permits the analysis of flagellin specifically for its NLRC4 stimulating activity. Binding of IL-1β to IL-1R initiates a signaling cascade leading to the activation of NF-kB and the subsequent production of SEAP.
Detection of SEAP in the supernatant of HEK-Blue™ KD-TLR5 cells can be readily assessed using the QUANTI-Blue™ assay. Levels of IL-1β secreted into the supernatant of THP-1 cells can be monitored using the HEK-Blue™ KD-TLR5 cell line.
THP1-NLRC4 and HEK-Blue™ KD-TLR5 cell lines can be used sequentially (option 1) or co-cultured to save time (option 2).
THP-1/HEK-Blue™ IL-1β Assay :
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