THP1-Dual™ KO-SAMHD1 Cells
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Cat.code:
thpd-kosamhd1
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ABOUT
SAMHD1 knockout NF-κB-SEAP and IRF-Lucia luciferase reporter monocytes
THP1-Dual™ KO-SAMHD1 cells were generated from THP1-Dual™ cells through the stable biallelic knockout of the SAMHD1 gene. Human THP1 monocytes or derived macrophages are a common cellular model to study DNA sensing as they naturally express all cytosolic DNA sensors identified so far (except DAI). THP1-Dual™ KO-SAMHD1 cells feature two inducible reporter genes, allowing the concomitant study of the IRF and NF-κB pathways, by monitoring the Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase) activities, respectively.
SAMHD1 (sterile alpha motif and histidine-aspartate domain-containing protein 1) is a predominantly nuclear enzyme playing a major role in nucleotide homeostasis by balancing cellular dNTP (deoxynucleoside triphosphate) levels [1]. Furthermore, it can influence viral activity and act as an important negative regulatory factor of the human innate immune response [1-3].
Key features:
- Biallelic knockout of the SAMHD1 gene
- Functionally validated with a selection of PRR ligands and cytokines
- Readily assessable Lucia luciferase and SEAP reporter activities
Applications:
- Study of IRF and NF-kB-dependent SAMHD1 signaling pathways
- Screening of interactions between SAMHD1 and other signaling protein
- Study the role of SAMHD1 in innate immunity, tumorigenesis, or viral replication
1. Deutschmann et al., 2021. SAMHD1 … and Viral Ways around It. Viruses. 2021;13(3):395.
2. Rice et al., 2009. Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as a regulator of the innate immune response. Nature genetics, 41(7), 829–832.
3. Oo et al., 2022. Elimination of Aicardi–Goutières syndrome protein SAMHD1 activates cellular innate immunity and suppresses SARS-CoV-2 replication. jbc.2022.101635
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
SAMHD1
Human
Complete RPMI 1640 (see TDS)
Verified using Plasmotest
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:THP1-Dual™ KO-SAMHD1 Cells
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Cat code:thpd-kosamhd1
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Quantity:3-7 x 10^6 cells
- 1 ml of Normocin™ (50 mg/ml).
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Blasticidin (10 mg/ml)
- 1 tube of QUANTI-Luc™ 4 Reagent (sufficient to prepare 25 ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution)
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
- Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.
Storage:
Caution:
Details
SAMHD1 plays a variety of roles in cell biology, immunology, and virology [1].
Using its phosphohydrolase activity, SAMHD1 converts dNTPs into inorganic triphosphates (PPPis) and deoxynucleosides (dN) to ensure efficient genome replication in dividing cells [2]. Moreover, it facilitates the replication fork progression and prevents DNA damage by actively recruiting members of the DSB (double-strand break) repair machinery [1].
Additionally, SAMHD1 can interfere with mediators of NF-κB and IRF signaling pathways, thus preventing an excessive antiviral and proinflammatory response [2]. Depending on whether a virus benefits from these suppressive effects on the host immune system, SAMHD1 allows for either antiviral or proviral events. SAMHD1 has been reported to limit HIV-1 replication while promoting Zika or SARS-CoV-2 infection [3].
Defects or alterations in the SAMHD1 gene have been associated with tumor development and Aicardi-Goutières syndrome, an autoimmune disorder characterized by spontaneous hyperactivation of the type I IFN pathway and excessive IFN-α production. This syndrome is also induced by mutations in other nuclease-coding genes such as TREX1 or RNASEH2 [3,4].
1. Coggins et al., 2020. SAMHD1 Functions and Human Diseases. Viruses, 12(4), 382.
2. Deutschmann et al., 2021. SAMHD1 … and Viral Ways around It. Viruses.13(3):395.
3. Oo et al., 2022. Elimination of Aicardi–Goutières syndrome protein SAMHD1 activates cellular innate immunity and suppresses SARS-CoV-2 replication. jbc.2022.101635.
4. Rice et al., 2009. Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as a regulator of the innate immune response. Nature genetics, 41(7), 829–832.
DOCUMENTS
Documents
Validation Data Sheet
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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