THP1-Dual™ KO-TREX1 Cells
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Cat.code:
thpd-kotrex
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ABOUT
TREX1 knockout NF-κB-SEAP and IRF-Lucia luciferase reporter monocytes
THP1-Dual™ KO-TREX1 cells were generated from THP1-Dual™ cells by stable biallelic knockout of the TREX1 gene. Human THP1 monocytes or derived macrophages are a common cellular model to study DNA sensing as they naturally express all cytosolic DNA sensors identified so far (except DAI). THP1-Dual™ KO-TREX1 cells feature two inducible reporter genes allowing the concomitant study of the IRF and NF-κB pathways, by monitoring the Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase) activities, respectively.
TREX1 (three prime repair exonuclease 1) is a major cellular 3'->5' exonuclease that plays a crucial role in maintaining immune homeostasis [1,2].
Key features:
- Biallelic knockout of the TREX1 gene
- Functionally validated with a selection of PRR ligands and cytokines
- Readily assessable Lucia luciferase and SEAP reporter activities
Applications:
- Study of IRF and NF-κB-dependent TREX1 signaling pathways
- Screening of interactions between TREX1 and other signaling protein
- Study the role of TREX1 in innate immunity
1. Kavanagh D. et al., 2008. New roles for the major human 3'-5' exonuclease TREX1 in human disease. Cell Cycle. 7(12):1718-25.
2. Hasan M. & Yan N., 2014. Safeguard against DNA sensing: the role of TREX1 in HIV-1 infection and autoimmune diseases. Front Microbiol. 5:193.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
TREX1
Human
Complete RPMI 1640 (see TDS)
Verified using Plasmotest™
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:THP1-Dual™ KO-TREX1 Cells
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Cat code:thpd-kotrex
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Quantity:3-7 x 10^6 cells
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Blasticidin (10 mg/ml)
- 1 tube of QUANTI-Luc™ 4 Reagent (sufficient to prepare 25 ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution)
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
- Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.
Storage:
Caution:
Details
TREX1 (also known as DNase III) is a major DNA-sensor nuclease in the cytoplasm.
The primary role of TREX1 is to target cellular DNA originating from aberrant replication and recombination [1]. TREX1 is bound to the ER (endoplasmic reticulum) and is able to degrade both single-stranded and double-stranded DNA as well as single-strand RNA. As a result, it blocks the activation of the cGAS-STING pathway, and thus dampening the nucleic acid sensor response. Therefore, it is thought to be a negative regulator of interferon (IFN) signaling preventing autoimmune diseases. Its acidic counterpart, DNase2, shares the same function, but is located in the lysosomes [2].
However, TREX1 function also promotes protumor and -viral responses by degrading tumor- or virus-derived DNA that would otherwise stimulate the cGAS-STING pathway and elicit an immune response [2-3]. Unlike SAMHD1, another enzyme with nuclease activity, TREX1 boosts HIV-1 infection [4]. Mutations in TREX1 have been associated with a variety of disorders, such as the Aicardi–Goutières syndrome, Systemic Lupus Erythematosus or hereditary vascular retinopathy [1-4].
1. Kavanagh D. et al., 2008. New roles for the major human 3'-5' exonuclease TREX1 in human disease. Cell Cycle. 7(12):1718-25.
2. Baris, Adrian M et al., 2021. “Nucleic Acid Sensing in the Tumor Vasculature.” Cancers vol. 13,17 4452.
3. Hemphill et al., 2021. TREX1 as a Novel Immunotherapeutic Target. Front Immunol. Apr 1;12:660184.
4. Hasan M. & Yan N., 2014. Safeguard against DNA sensing: the role of TREX1 in HIV-1 infection and autoimmune diseases. Front Microbiol. 5:193
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Validation Data Sheet
Certificate of analysis
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