THP1-Dual™ KO-IRF7 Cells
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Cat.code:
thpd-koirf7
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ABOUT
IRF7 knockout dual reporter monocytes
THP1-Dual™ KO-IRF7 cells were generated from the THP1-Dual™ cell line through the stable knockout of the IRF7 gene. They feature two inducible reporter genes, allowing the concomitant study of the IRF and NF-κB pathways, by monitoring the Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase) activities, respectively. We observe an impact of the IRF-7 knock-out on NF-κB-mediated responses, and no effect on IRF-mediated responses, in THP1-Dual™ KO-IRF7 cells upon incubation with TLR agonists when compared to the THP1-Dual™ cells (see Figures).
Interferon regulatory receptor 7 (IRF7) is a transcription factor involved in the activation of the type I interferon (IFN) response upon pathogenic infection [1-2]. IRF7 has been implicated downstream of several pattern recognition receptors (PRRs), including TLRs, RLRs, and DNA sensors [2]. IRF7 is highly homologous to IRF3, and they can form either homo- or heterodimers, which ultimately lead to IFN production [2].
Key Features:
- Verified knockout of the IRF7& gene (PCR, DNA sequencing, and Western blot)
- Functionally validated with a selection of PRR ligands and cytokines
- Readily assessable Lucia luciferase and SEAP reporter activities
Applications:
- Defining the role of IRF7 in PRR-induced signaling, or other cell signaling pathways
- Highlighting the possible functional overlap between IRF7 and other transcriptional factors
- Distinguishing the overlapping and differing roles of IRF7 and IRF3 in various signaling pathways
References
1. Ning, S. et al. 2011. IRF7: activation, regulation, modification and function. Genes Immun 12, 399-414.
2. Perrotti, E. et al. 2013. IRF-7: an antiviral factor and beyond. Future Virol. 8(10): 1007–1020.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
Screening of PRR agonists or inhibitors
Complete RPMI 1640 (see TDS)
Verified using Plasmotest™
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:THP1-Dual™ KO-IRF7 Cells
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Cat code:thpd-koirf7
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Quantity:3-7 x 10^6 cells
- 1 ml of Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria, and fungi.
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Blasticidin (10 mg/ml)
- 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
Storage:
Details
IRF7 background
Interferon regulatory factor 7 (IRF7) is a transcription factor involved in the activation of the type I IFN response upon pathogenic infection [1, 2]. IRF7 is constitutively expressed by plasmacytoid dendritic cells (pDCs), which are known as ‘professional’ type I IFN producing cells [1]. However, in all other cell types IRF7, unlike its closest homolog IRF3, is only expressed upon pathogenic infection [1, 2]. IRF7 has been implicated downstream of several pattern recognition receptors (PRRs), such as TLRs, RLRs, and DNA sensors [2]. Upon PRR stimulation, IRF7 is phosphorylated through the action of the IKK-related kinases TBK1 and IKKε, and forms a homo- or heterodimer with IRF3, ultimately leading to IFN production [2]. Despite both IRF3 and IRF7 being required for IFN production in most immune cells, they have been shown to also have distinct and unique features. IRF7 is solely responsible for the production of IFN-α [1]. Importantly, IRF7 is part of a positive feedback regulatory loop essential for sustained IFN responses and full protective adaptive immunity [2]. Furthermore, it has been established that IRF7 is involved in other cellular functions, including the regulation of oncogenesis [1, 2].
1. Ning, S. et al. 2011. IRF7: activation, regulation, modification and function. Genes Immun 12, 399-414.
2. Perrotti, E. et al. 2013. IRF-7: an antiviral factor and beyond. Future Virol. 8(10): 1007–1020.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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