Engineered Human Reporter A549 Lung Carcinoma Cells
InvivoGen provides a collection of A549-derived lung carcinoma cell lines. A549 human lung epithelial cells were originally isolated from the alveolar basal epithelial tissue of a male patient with non-small cell lung cancer (NSCLC). This adherent growing cell line has become a type II lung epithelial cell model for studying lung adenocarcinomas, asthma, allergies, as well as respiratory infections (e.g. COVID-19). A549 cells express numerous pattern recognition receptors (PRRs), including RIG-I-like receptors (RLRs), Toll-like receptors (TLRs), and various components of the inflammasome complex.
Therefore, A549 cells are a great tool to:
- study respiratory diseases or lung cancer
- investigate cell signaling pathways or inflammasome assembly
- screen for agonists/antagonists of relevant PRRs
- unravel the mysteries surrounding inflammasome activation
InvivoGen has generated several groups of A549-derived cells that allow (1) the monitoring of the NF-κB and IRF pathway activation upon TLR or RLR activation, (2) the monitoring of ASC-dependent inflammasome activation by visualizing GFP specks using fluorescence microscopy, (3) the study of SARS-CoV2 entry through ACE2 and TMPRSS2 receptors and (4) tetracycline-inducible expression of a gene of interest (GOI).
A549-Dual™ reporter cells feature two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase. As a result, these cells allow the simultaneous study of the NF-κB and/or the IRF pathways, by assessing the activity of SEAP or the secreted Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent.
A549 inflammasome reporter cells feature an NF-κB-inducible ASC::GFP reporter gene to allow the monitoring of ASC-dependent inflammasome activation by visualizing GFP specks using fluorescence microscopy.
SARS-CoV-2-related A549 cells stably overexpress one or two genes encoding for the SARS-CoV-2 receptors, human ACE2, and TMPRSS2. In addition, they can either feature the Dual™ reporter proteins SEAP and Lucia, or an ASC::GFP fusion reporter protein.
Tet-on inducible A549-RepTor™ cells stably and constitutively express an optimized tetracycline repressor (TetR) construct. They are designed for the tetracycline-inducible expression of a gene of interest (GOI), including cytotoxic proteins.
All our cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end-user.
Key Features
- Verified overexpression and/or knockout of important PRRs
- Stable expression of one or two reporter systems
- Quantifiable responses upon stimulation with pathogen/damage-associated molecular patterns (PAMPs/DAMPs)
- Functionally tested and guaranteed mycoplasma-free
Fields of Interest
- RIG-I-like Receptors (RLRs)
- Inflammasomes
- COVID-19
- Tet-inducible protein expression
