|QUANTI-Blue™ Solution||Unit size||Cat. code||Docs||Qty||Price|
Alkaline phosphatase detection medium - Liquid form
5 ml (100X)
10 ml (100X)
|QUANTI-Blue™||Unit size||Cat. code||Docs||Qty||Price|
Alkaline phosphatase detection medium - Powder
5 pouches (5 x 100 ml)
10 pouches (10 x 100 ml)
QUANTI-Blue™ is a colorimetric enzyme assay developed to determine any alkaline phosphatase (AP) activity in a biological sample, such as cell culture supernatant.
InvivoGen provides a wide collection of reporter cell lines with the secreted embryonic alkaline phosphatase (SEAP) reporter gene encoding a truncated form of placental AP.
SEAP secretion in cell culture supernatant presents multiple advantages over intracellular reporters.
QUANTI-Blue™ Solution is a liquid formulation (100X) of QUANTI-Blue™ Powder (1X) offering a convenient, easy and rapid means to detect and quantify SEAP and AP activity.
- Short hands-on-time: < 10 min
- Easy to prepare: just mix QB Reagent and QB Buffer with water
- Highly sensitive
- Wide dynamic range
- High saturation threshold
- Allows high-throughput screening (HTS)
In the presence of AP, the color of QUANTI-Blue™ changes from pink to purple/blue. The purple/blue color intensity reflects the activity of AP.
The levels of AP can be determined qualitatively with the naked eye or quantitatively using a spectrophotometer at 620-655 nm.
Physicochemical characterization: pH and solubility
Functional characterization: cellular assays using alkaline phosphatase of SEAP-expressing reporter cells.Back to the top
QUANTI-Blue™ Solution is available in two pack sizes:
- rep-qbs : 5 x 1 ml of QB reagent + 5 x 1 ml QB buffer, sufficient to prepare 500 ml of QUANTI-Blue™.
- rep-qbs2 : 10 x 1 ml of QB reagent + 10 x 1 ml QB buffer, sufficient to prepare 1 liter of QUANTI-Blue™.
Store QB reagent and QB buffer at -20°C.
Reconstituted QUANTI-Blue™ Solution is stable for 2 weeks at 2-8 °C and for 2 months at -20°C. Keep reconstituted QUANTI-Blue™ away from light.
QUANTI-Blue™ Powder is provided as packs of individually sealed pouches:
- rep-qb1: 5 pouches (5 x 100 ml).
- rep-qb2: 10 pouches (10 x 100 ml).
Each pouch allows the preparation of 100 ml of QUANTI-Blue™.
QUANTI-Blue™ is shipped at room temperature.
Store QUANTI-Blue™ pouches at 2-8°C.
Reconstituted QUANTI-Blue™ medium is stable for 2 weeks at 2-8 °C and for 2 months at -20°C. Keep reconstituted QUANTI-Blue™ away from light.Back to the top