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QUANTI-Blue™ [ NEW ]

QUANTI-Blue™ Solution Unit size Cat. code Docs Qty Price
Alkaline phosphatase detection medium - Liquid form
5 ml
10 ml
rep-qbs
+-
$164.00
QUANTI-Blue™ Unit size Cat. code Docs Qty Price
Alkaline phosphatase detection medium - Powder
5 pouches (5 x 100 ml)
10 pouches (10 x 100 ml)
rep-qb1
+-
$164.00

QUANTI-Blue™ Solution | InvivoGen

NEW PRODUCT : QUANTI-Blue™ Solution

 

QUANTI-Blue™ is a colorimetric enzyme assay developed to determine any alkaline phosphatase (AP) activity in a biological sample, such as cell culture supernatant.

InvivoGen provides a wide collection of reporter cell lines with the secreted embryonic alkaline phosphatase (SEAP) reporter gene encoding a truncated form of placental AP.

SEAP secretion in cell culture supernatant presents multiple advantages over intracellular reporters.

 

QUANTI-Blue™ Solution is a liquid formulation of QUANTI-Blue™ Powder offering a convenient, easy and rapid means to detect and quantify SEAP and AP activity.

  • Short hands-on-time: < 10 min
  • Easy to prepare: just mix QB Reagent and QB Buffer with water
  • Highly sensitive
  • Wide dynamic range
  • High saturation threshold
  • Allows high-throughput screening (HTS)

In the presence of AP, the color of QUANTI-Blue™ changes from pink to purple/blue. The purple/blue color intensity reflects the activity of AP.
The levels of AP can be determined qualitatively with the naked eye or quantitatively using a spectrophotometer at 620-655 nm.

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Specifications

Physicochemical characterization: pH and solubility

Functional characterization: cellular assays using alkaline phosphatase of SEAP-expressing reporter cells.

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Contents

QUANTI-Blue™ Solution is available in two pack sizes:

  • rep-qbs : 5 x 1 ml of QB reagent + 5 x 1 ml QB buffer, sufficient to prepare 500 ml of QUANTI-Blue™.
  • rep-qbs2 : 10 x 1 ml of QB reagent + 10 x 1 ml QB buffer, sufficient to prepare 1 liter of QUANTI-Blue™.

Store QB reagent and QB buffer at -20°C.

Reconstituted QUANTI-Blue™ Solution is stable for 2 weeks at 2-8 °C and for 2 months at -20°C. Keep reconstituted QUANTI-Blue™ away from light.

 

 

QUANTI-Blue™  Powder is provided as packs of individually sealed pouches:

  • rep-qb1: 5 pouches (5 x 100 ml).
  • rep-qb2: 10 pouches (10 x 100 ml).

Each pouch allows the preparation of 100 ml of QUANTI-Blue™.

QUANTI-Blue™ is shipped at room temperature.

Store QUANTI-Blue™ pouches at 2-8°C.

Reconstituted QUANTI-Blue™ medium is stable for 2 weeks at 2-8 °C and for 2 months at -20°C. Keep reconstituted QUANTI-Blue™ away from light.

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Citations

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