SEAP Gene

SEAP Reporter Gene in expression plasmid

ABOUT

SEAP Reporter Gene in expression plasmid

InvivoGen provides the secreted embryonic alkaline phosphatase (SEAP) gene in the pSELECT-zeo-SEAP plasmid. It can be used in vivo and in vitro to transfect mammalian cells stably or transiently.

The SEAP gene expression is driven by the EF-1α/HTLV composite promoter that combines the elongation factor 1 alpha core promoter and the 5’untranslated region of the Human T-cell Leukemia Virus.

The pSELECT-zeo-SEAP plasmid is selectable with Zeocin™ in both mammalian cells and bacteria.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

Gene expression with selectable markers

Gene
SEAP
ORF size
1563 bp
Plasmid backbone
pSELECT-zeo
Antibiotic resistance
Zeocin®
Gene promoter
Human EF1α/HTLV hybrid promoter
Purification
Ion-exchange chromatography
Appearance (form)
Lyophilized
Reconstitution buffer
Sterile water (not provided)
Tested applications

in vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing.

CONTENTS

Contents

  • Product: 
    pSELECT-zeo-SEAP
  • Cat code: 
    psetz-seap
  • Quantity: 
    20 µg
Includes:
  • 1 ml of Zeocin™ (100 mg/ml)
  • 1 ml of QB reagent
  • 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution)

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

DOCUMENTS

Documents

pSELECT-zeo-SEAP

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

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