Recombinant SEAP Protein
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Recombinant secreted embryonic alkaline phosphatase protein
Positive control for SEAP reporter assays
Recombinant SEAP (secreted embryonic alkaline phosphatase) protein is a truncated form of human placental alkaline phosphatase that comprises 520 amino acids.
It is expressed in CHO cells and shows a 75 kDa band on SDS page. Recombinant SEAP protein is purified by affinity chromatography.
For research use only, not for diagnostic or therapeutic use.Back to the top
Formulation: SEAP protein is formulated in 91 mM glycine, 91 mM TRIS and 5% w/v saccharose.
Purity : > 90% determined by SDS-PAGE Coomassie Blue under denaturing conditions
Applications: Positive control for SEAP reporter assays.
A dilution series of the recombinant SEAP protein can also be used to determine the linear range of the assays.
10 μg lyophilized recombinant human secreted embryonic alkaline phosphatase (SEAP).
Recombinant SEAP Protein is shipped at room temperature.
Upon receipt it should be stored -20°C for 12 months.Back to the top
Activity of recombinant SEAP protein determined by measuring the OD at 630 nm using QUANTI-Blue™. Values were measured after 1h in QUANTI-Blue™.Back to the top