Recombinant SEAP Protein

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Recombinant SEAP Protein

Recombinant secreted embryonic alkaline phosphatase protein

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10 µg


Positive control for SEAP reporter assays

Recombinant SEAP (secreted embryonic alkaline phosphatase) protein is a truncated form of human placental alkaline phosphatase that comprises 520 amino acids.

It is expressed in CHO cells and shows a 75 kDa band on SDS page. Recombinant SEAP protein is purified by affinity chromatography.


For research use only, not for diagnostic or therapeutic use.

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Formulation: SEAP protein is formulated in 91 mM glycine, 91 mM TRIS and 5% w/v saccharose.

Purity : > 90% determined by SDS-PAGE Coomassie Blue under denaturing conditions

Applications: Positive control for SEAP reporter assays.
A dilution series of the recombinant SEAP protein can also be used to determine the linear range of the assays.

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10 μg lyophilized recombinant human secreted embryonic alkaline phosphatase (SEAP).


Recombinant SEAP Protein is shipped at room temperature.

Upon receipt it should be stored -20°C for 12 months.

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Activity of recombinant SEAP protein

Activity of recombinant SEAP protein determined by measuring the OD at 630 nm using QUANTI-Blue™. Values were measured after 1h in QUANTI-Blue™.

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