Engineered Human Reporter HCT116 Colorectal Carcinoma Cells
InvivoGen provides an HCT116-derived dual reporter cell line. HCT116 was originally isolated from the colon of a patient with colon cancer. This human colorectal carcinoma cell line is adherent with an epithelial morphology. It has a relatively stable genetic profile and thus is a valuable in vitro model for cancer research and gastrointestinal research.
InvivoGen has developed HCT116-Dual cells designed for the study of the NF-κB and/or IRF pathways. This cell line features two inducible reporter constructs allowing the simultaneous monitoring of NF-κB and IRF activation by assessing the activity of secreted embryonic alkaline phosphatase (SEAP) and the secreted Lucia luciferase, respectively. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent.
This cell line is extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for this cell line, to minimize the need for optimization or troubleshooting by the end-user.
Key Features
- Stable expression of two reporter systems
- Quantifiable responses upon stimulation with pathogen/damage-associated molecular patterns (PAMPs/DAMPs) or cytokines
- Functionally tested and guaranteed mycoplasma-free
Fields of Interest
- Gastrointestinal research
- Colon cancer research
