Engineered Human Reporter HCT116 Colorectal Carcinoma Cells
InvivoGen provides HCT116-derived reporter cell lines for cancer, gastrointestinal, and cell signaling research. HCT116 was originally isolated from the colon of a patient with colon cancer. This human colorectal carcinoma cell line is adherent with an epithelial morphology. It has a relatively stable genetic profile and thus is a valuable in vitro model for cancer research and gastrointestinal research.
InvivoGen has developed HCT116-Dual cells designed for the study of the NF-κB and/or IRF pathways. This cell line features two inducible reporter constructs allowing the simultaneous monitoring of NF-κB and IRF activation by assessing the activity of secreted embryonic alkaline phosphatase (SEAP) and the secreted Lucia luciferase, respectively. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent.
InvivoGen also offers HCT116-Lucia™ AhR cells, designed for the quantitative study of aryl hydrocarbon receptor (AhR) signaling. These cells express endogenous AhR and stably express an AhR-inducible Lucia® luciferase reporter, enabling sensitive monitoring of AhR activation.
These cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures to minimize the need for optimization or troubleshooting by the end-user.
Key Features
- Stable expression of inducible reporter systems for NF-κB/IRF or AhR signaling
- Quantifiable responses upon stimulation with pathway-specific agonists
- Functionally tested and guaranteed mycoplasma-free
Fields of Interest
- Gastrointestinal research
- Colon cancer research
- Gut microbiota and host interactions
- Intestinal AhR signaling
