Ubiquitous Promoters

Ubiquitous Promoters are strongly active in a wide range of cells, tissues and cell cycles.

Ubiquitous Promoters are available as native or composite promoter:

  • Native promoters, also called minimal promoters,  consist of a single fragment from the 5’ region of a given gene. Each of  them comprises a core promoter and its natural 5’UTR. In some cases,  the 5’UTR contains an intron.
  • Composite promoters combine promoter elements of different origins (e.g. SV40 enhancer/AFP  promoter) or were generated by assembling a distal enhancer with a  minimal promoter of the same origin (e.g. CEA enhancer/promoter).

In each pDRIVE, the promoter drives the expression of a reporter gene to  facilitate the evaluation of its activity.

Three different reporter genes  are available in two different backbones: LacZ reporter gene in pDRIVE, secreted embryonic alkaline phosphatase (SEAP) or Lucia secreted luciferase reporter gene in pDRIVE5.

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