pDRIVE-HSP70

HSP70 promoter (Ubiquitous, heat inducible) in pDRIVE expression plasmid

ABOUT

HSP70 promoter (Ubiquitous, heat-inducible) in pDRIVE expression plasmid

Heat inducible

The HSP70 gene encodes a major stress-inducible heat shock protein (HSP70) which plays an important role in protecting cells from deleterious stresses. Hsp70 is expressed constitutively in a wide variety of cells at normal growth temperatures.

Its expression is induced by various physiological stresses, including exposure to heat shock and heavy metal, or under non-stress conditions, such as in response to serum stimulation.

The HSP70 promoter contains at least two regulatory domains, a distal domain responsive to heat shock or heavy metals and a proximal domain responsive to stimulation by serum [1].

The activity of the HSP70 promoter can be induced by moderate hyperthermia (39°C to 43°C) [2], reaching expression levels similar to those of the CMV promoter.

 

References:

1. Wu BJ. et al.,  1986. Human HSP70 promoter contains at least two distinct regulatory domains. Proc Natl Acad Sci USA. 83(3):629-33.
2. Huang Q. et al., 2000. Heat-induced gene expression as a novel targeted cancer gene therapy strategy. Cancer Res. 60(13):3435-9.

All products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

in vitro or in vivo transfection

Species
Human
Plasmid backbone
pDRIVE
Antibiotic resistance
Zeocin®
Gene promoter
HSP70
Specificity

Ubiquitous, heat inducible

Reporter gene
Lac Z
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Tested applications

in vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and full‑length ORF sequencing.

CONTENTS

Contents

  • Product: 
    pDRIVE-hHSP70
  • Cat code: 
    pdrive-hhsp70
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin® (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pDRIVE-hHSP70

Technical Data Sheet

Plasmid Map and Sequence

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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