pDRIVE-HSP70

HSP70 promoter (Ubiquitous, heat-inducible) in pDRIVE expression plasmid

Heat inducible

The HSP70 gene encodes a major stress-inducible heat shock protein (HSP70) which plays an important role in protecting cells from deleterious stresses. Hsp70 is expressed constitutively in a wide variety of cells at normal growth temperatures.

Its expression is induced by various physiological stresses, including exposure to heat shock and heavy metal, or under non-stress conditions, such as in response to serum stimulation.

The HSP70 promoter contains at least two regulatory domains, a distal domain responsive to heat shock or heavy metals and a proximal domain responsive to stimulation by serum [1].

The activity of the HSP70 promoter can be induced by moderate hyperthermia (39°C to 43°C) [2], reaching expression levels similar to those of the CMV promoter.

References:

1. Wu BJ. et al.,  1986. Human HSP70 promoter contains at least two distinct regulatory domains. Proc Natl Acad Sci USA. 83(3):629-33.
2. Huang Q. et al., 2000. Heat-induced gene expression as a novel targeted cancer gene therapy strategy. Cancer Res. 60(13):3435-9.