pDRIVE-hHSP70
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Cat.code:
pdrive-hhsp70
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ABOUT
HSP70 promoter (Ubiquitous, heat-inducible) in pDRIVE expression plasmid
Heat inducible
The HSP70 gene encodes a major stress-inducible heat shock protein (HSP70) which plays an important role in protecting cells from deleterious stresses. Hsp70 is expressed constitutively in a wide variety of cells at normal growth temperatures.
Its expression is induced by various physiological stresses, including exposure to heat shock and heavy metal, or under non-stress conditions, such as in response to serum stimulation.
The HSP70 promoter contains at least two regulatory domains, a distal domain responsive to heat shock or heavy metals and a proximal domain responsive to stimulation by serum [1].
The activity of the HSP70 promoter can be induced by moderate hyperthermia (39°C to 43°C) [2], reaching expression levels similar to those of the CMV promoter.
References:
1. Wu BJ. et al., 1986. Human HSP70 promoter contains at least two distinct regulatory domains. Proc Natl Acad Sci USA. 83(3):629-33.
2. Huang Q. et al., 2000. Heat-induced gene expression as a novel targeted cancer gene therapy strategy. Cancer Res. 60(13):3435-9.
All products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
in vitro or in vivo transfection
Ubiquitous, heat inducible
in vitro transfection
Plasmid construct has been confirmed by restriction analysis and full‑length ORF sequencing.
CONTENTS
Contents
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Product:pDRIVE-hHSP70
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Cat code:pdrive-hhsp70
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Quantity:20 µg
1 ml of Zeocin® (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Plasmid Map and Sequence
Plasmid Sequence
Safety Data Sheet
Certificate of analysis
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