CH-223191
| CH-223191 | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
2-methyl-2H-pyrazole-3-carboxylic acid; AhR Antagonist |
10 mg |
inh-ch22 |
AhR Antagonist
CH-223191 is a synthetic antagonist for the cytosolic aryl hydrocarborn receptor (AhR) transcription factor [1].
It exerts a ligand-selective antagonism and appears to be more effective on halogenated aromatic hydrocarbons such as the xenobiotic 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), than on polycyclic aromatic hydrocarbons and non halogenated aromatic hydrocarbons such as FICZ and ITE, respectively [2].
InvivoGen's CH-223191 is of high quality, guaranteed free of bacterial contamination, and has been functionally tested on HepG2-Lucia™ AhR and HT29-Lucia™ AhR reporter cells.
Read our review on AhR's key role in the intestinal microbiota and immunity.
References
1. Kim, S.H. et al., 2006. Novel compound 2-methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) prevents 2,3,7,8-TCDD-Induced toxicity by antagonizing the aryl hydrocarbon receptor. Mol. Pharmacol. 69:1871-78.
2. Zhao B. et al., 2010. CH223191 is a ligand-selective antagonist of the Ah (Dioxin) receptor. Toxicol. Sci. 117:393-403.
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Specifications
CAS number: 301326-22-7
Synonym: 2-methyl-2H-pyrazole-3-carboxylic acid
Solubility: 90 mM (30 mg/ml) in DMSO
Working concentration: 1µM (333 ng/ml) to 30 µM (10.1 μg/ml) for cell culture assays
Formula: C19H19N5O
Molecular weight: 333.4 g/mol
Quality control:
- Purity ≥ 95% (UHPLC).
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
- The inhibitory activity has been validated using cellular assays.
CH-223191 is shipped at room temperature.
Store at 4°C.