Rapamycin

mTOR Inhibitor and Autophagy Inducer

Rapamycin is an inhibitor of the Ser/Thr protein kinase mTOR (mammalian target of rapamycin), which regulates cell growth and metabolism in response to environmental cues.

Rapamycin is also an inducer of autophagy, as inhibition of mTOR mimics cellular starvation by blocking signals required for cell growth and proliferation [1].

Furthermore, mTOR is a downstream target of PI3K, an important actor in TLR signaling [2].

Indeed, Rapamycin can block TLR2- and TLR4-mediated expression of TNF-α and IL-6 in neutrophils stimulated with Pam3CSK4 or LPS [3].

References

1. Jung CH. et al., 2010. mTOR regulation of autophagy. FEBS Lett. 584(7):1287-95.
2. Kuo CC. et al., 2006. Class I and III Phosphatidylinositol 3'-Kinase Play Distinct Roles in TLR Signaling Pathway. J. Immunol., 176: 5943 - 5949.
3. Lorne E. et al., 2009. Participation of Mammalian Target of Rapamycin Complex 1 in Toll-Like Receptor 2– and 4–Induced Neutrophil Activation and Acute Lung Injury. Am. J. Respir. Cell Mol. Biol., 41: 237 - 245. 

Specifications

Working concentration:
- 10-100 nM when used as an inhibitor in the TLR signaling cascade
- 10-500 nM when used to induce autophagy

Purity:>95% (HPLC)

Endotoxin level: <0.125 EU/mg

Solubility: 10 mM in DMSO or 2 mM in ethanol

CAS number: 53123-88-9

Synonym: 23,27-Epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentriacontine, Antibiotic AY 22989; NSC 2260804; Sirolimus

Molecular weight: 914.17
 

Contents

Rapamycin is provided as a solid

• 5 mg Rapamycin

Rapamycin is shipped at room temperature.

Store at -20°C.
Upon resuspension, Rapamycin should be aliquoted and stored at -20°C.

Solid product is stable 1 year at -20°C.
Resuspended product is stable fo 3 months at -20°C when properly stored.

Avoid repeated freeze-thaw cycles.