|Z-VAD-FMK||Unit size||Cat. code||Docs||Qty||Price|
Z-VAD-FMK is a cell-permeable pan-caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases . The peptide is O-methylated in the P1 position on aspartic acid, providing enhanced stability and increased cell permeability.
Importantly, Z-VAD-FMK is used inflammasome studies, as it is a potent inhibitor of caspase-1 activation in NLRP3-induced cells . Interestingly, through the inhibition of caspases 3, 7 and 8, Beclin1 is stabilized and autophagy is promoted by Z-VAD-FMK.
1. Slee EA. et al., 1996. Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32. Biochem J. 315 ( Pt 1):21-4.
2. Dostert C. et al., 2009. Malarial hemozoin is a Nalp3 inflammasome activating danger signal. PLoS One. 4(8):e6510.
Read our latest review on the NLRP3 inflammasomeBack to the top
Working concentration: 10 μg/ml (20 μM)
Molecular weight: 467.5
Solubility: Soluble in DMSO (20 mM/10 mg/ml)Back to the top
Z-VAD-FMK is provided as a white solid.
- 1 mg of Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O- methyl]- fluoromethylketone)
Z-VAD-FMK is shipped at room temperature.
Store at -20 ̊C.
Solid product is stable 1 year at -20°C.Back to the top