Chloroquine

Endosomal Acidification Inhibitor

Chloroquine is a lysosomotropic agent that prevents endosomal acidification [1]. It accumulates inside the acidic parts of the cell, including endosomes and lysosomes.

This accumulation leads to inhibition of lysosomal enzymes that require an acidic pH, and prevents fusion of endosomes and lysosomes.

Chloroquine is commonly used to study the role of endosomal acidification in cellular processes [2, 3], such as the signaling of intracellular TLRs.

Moreover, Chloroquine inhibits autophagy as it raises the lysosomal pH, which leads to inhibition of both fusion of autophagosome with lysosome and lysosomal protein degradation [4].

References

1. Steinman RM. et al., 1983. Endocytosis and the recycling of plasma membrane. J. Cell. Biol. 96:1-27.
2. Rutz, M. et al., 2004. Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner. Eur. J. Immunol. 34:2541-2550.
3. Hart OM. et al., 2005. TLR7/8-Mediated Activation of Human NK Cells Results in Accessory Cell-Dependent IFN-{gamma} Production. J. Immunol., 175: 1636 - 1642.
4. T. Shintani and D.J. Klionsky, 2004. Autophagy in Health and Disease: A Double-Edged Sword. Science, 306(5698) : 990-995.

Specifications

Working concentration: 10 μM

CAS number: 50-63-5

Synoym: N4-(7-Chloro-4-quinolinyl)-N1,N1-dimethyl-1,4- pentanediamine diphosphate salt

Solubility: Water (50 mg/ml)

Molecular weight: 515.86 g/mol

Quality control:

• Purity: ≥98% (UHPLC)
• The inhibitory activity has been validated using cellular assays.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ hTLR2 and HEK-Blue™ hTLR4 cells.

Contents

• 250 mg Chloroquine (diphosphate salt)

Chloroquine is shipped at room temperature.

Store at room temperature (15-25°C). Protect from light.

Product in powder form is stable for 6 months at room temperature when properly stored.