NLRP3 inflammasome inhibitor - MCC950

MCC950 Unit size Cat. code Docs Qty Price
NLRP3 inflammasome inhibitor
10 mg

NLRP3 inflammasome inhibitor

Inhibition of the NLRP3 inflammasome by MCC950
Inhibition of the NLRP3 inflammasome by MCC950

MCC950 is a potent and specific inhibitor of the NLRP3 (NOD-like receptor pyrin domain-containing protein 3, cryopyrin, or NALP3) inflammasome [1,2]. The multiprotein NLRP3 inflammasome complex is a key player in innate immunity. It participates in the production of the pro-inflammatory cytokines, interleukin-1β (IL-1β) and IL-18, as well as leading to alarmin secretion and pyroptosis, a form of immunogenic cell death.

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Mode of action:

MCC950 blocks the release of IL‑1β induced by NLRP3 activators, such as nigericin, ATP, and MSU crystals [3,4]. It inhibits the NLRP3 inflammasome by directly targeting the NLRP3 NATCH domain and interfering with the Walker B motif function thus preventing NLRP3 conformational change and oligomerization [1,2]. In research models of inflammation, such as cryopyrin-associated periodic syndromes (CAPS) and myocardial infarction, MCC950 effectively inhibited NLRP3-induced IL-1β production [3-5]. Importantly, MCC950 is a specific inhibitor of the NLRP3 inflammasome.  It displays no effect on the AIM2, NLRC4, or NLRP1 inflammasomes [4].

Key features:

  • A potent and specific inhibitor of the NLRP3 inflammasome
  • Each lot is highly pure (≥98%) and functionally tested


Read our review on the NLRP3 inflammasome.


Download our Practical guide on Inflammasomes.



1. Tapia-Abellán A. et al., 2019. MCC950 closes the active conformation of NLRP3 to an inactive state. Nature Chemical Biology. 15(6):560-4.
2. Coll R.C. et al., 2019. MCC950 directly targets the NLRP3 ATP-hydrolysis motif for inflammasome inhibition. Nature Chemical Biology. 15(6):556-9.
3. Guo H. et al., 2015. Inflammasomes: mechanism of action, role in disease, and therapeutics. Nat Med. 21(7):677-87.
4. Coll RC. et al., 2015. A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases. Nature Med 21(3), 248-255.
5. van Hout GP. et al., 2016. The selective NLRP3-inflammasome inhibitor MCC950 reduces infarct size and preserves cardiac function in a pig model of myocardial infarction. Eur Heart J. ehw247.




MCC950 dose-dependent inhibition of NLRP3 inflammasome response in monocytes
MCC950 dose-dependent inhibition of NLRP3 inflammasome response in monocytes

MCC950 inhibits the NLRP3 inflammasome response in a d ose-dependent manner.
THP1-Null2 cells, primed with LPS-EK (1 μg/ml for 3 h), were stimulated with MSU (150 µg/ml) and increasing concentrations of MCC950. After overnight incubation, IL-1β secretion was analyzed by adding 50 μl of supernatant from treated THP1-Null2 cells to HEK‑Blue™ IL‑1β cells. IL‑1β‑induced activation of NF‑κB was assessed by measuring the levels of SEAP in the supernatant of HEK-Blue™ IL-1β cells using QUANTI‑Blue™ Solution, a SEAP detection reagent, and by reading the optical density (OD) at 655 nm. Data are shown as a percentage (%) inhibition of the maximal response for the ligand with no inhibitor.

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CAS number: 210826-40-7

Working concentration: 300 nM (121.34 ng/ml) to 10 μM (4.04 μg/ml) for cell culture assays

Solubility: DMSO (10 mg/ml)

Formula: C20H24N2O5S

Molecular weight: 404.48 g/mol

Quality control:

  • Purity: ≥98% (UHPLC)
  • The inhibitory activity of the product has been validated in inflammasome cellular assays using THP1-Null2 and HEK-Blue™ IL-1β cells.
  • The absence of bacterial contamination (e.g. lipoproteins and endotoxins) is confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
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  • 10 mg MCC950 (provided as a translucent film)

MCC950 is shipped at room temperature.

Upon receipt, store at -20 °C.

Resuspended product is stable for at least 6 months when properly stored.

Alert Avoid repeated freeze-thaw cycles.

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The NLRP3 Inflammasome:

The NLRP3 inflammasome is an innate immune sensor that is activated by a two-step process; a first signal (‘priming’) provided mainly by bacterial components or endogenous cytokines involves NF-κB induction, while the second signal provided by a wide array of stimuli including microbial toxins, endogenous molecules or crystalline substances and leads to inflammasome assembly and activation [1, 2].  This triggers inflammasome multimerization and caspase-1 activation with the subsequent cleavage of interleukin-1β (IL-1β)/IL-18 and the pore-forming protein Gasdermin D (GSDMD) into their active forms. Additionally, the activation of the inflammasome also leads to alarmin secretion and pyroptosis, a form of immunogenic cell death.


1. Swanson K.V. et al., 2019. The NLRP3 inflammasome: molecular activation and regulation to therapeutics. Nat. Rev. Immunol. 19:477.
2. Groslambert M. & Py B. 2018. Spotlight on the NLRP3 inflammasome pathway. J. Inflamm. Res. 11:359.


Chemical structure of MCC950:

MCC950 chemical structure

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