RU.521
RU.521 | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Murine cGAS inhibitor |
2 mg |
inh-ru521 |
Specific inhibitor of murine cGAS
RU.521 is described as a small molecule inhibitor of cGAS (cyclic GMP-AMP synthase, cGAMP synthase) [1,2].
In a healthy individual, cGAS senses the abnormal presence of cytosolic DNA such as during bacterial or viral infection. In some autoimmune diseases, cGAS can be constitutively activated by mislocalized self-DNA leading to high type I interferon (IFN) production. RU.521 has been demonstrated to reduce IFN production in vitro and ex vivo in models of autoimmune diseases, such as Aicardi-Goutières [1,2]. RU.521 more potently inhibits the activity of murine cGAS compared to human cGAS [2].
Read our review on cytosolic DNA sensors.
References:
1. Vincent J. et al., 2017. Small molecule inhibition of cGAS reduces interferon expression in primary macrophages from autoimmune mice. Nat Commun. 28(1):750.
2. Zhou W. et al., 2018. Structure of the human cGAS-DNA complex reveals enhanced control of immune surveillance. Cell. 174(2):300-311.
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Specifications
Working concentration: 200 ng/ml (482 nM) to 20 μg/ml (48.2 μM) for cell culture assays.
Solubility: 5 mg/ml (12.05 mM) in DMSO
Formula: C19H12Cl2N4O3
Molecular weight: 415.23 g/mol
Quality control:
- Purity ≥ 95% (UHPLC).
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
- The inhibitory activity has been validated using cellular assays.
Contents
- 2 mg RU.521
RU.521 is shipped at room temperature.
Store at -20°C.
Resuspended product is stable for at least 6 months when properly stored.