ODN TTAGGG (A151)
ODN TTAGGG (A151) | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
TLR9 antagonist - Human preferred |
200 µg 1 mg |
tlrl-ttag151 |
Suppressive oligonucleotide - TLR9, AIM2 and cGAS antagonist
ODN TTAGGG (A151), also known simply as A151, is a synthetic oligonucleotide (ODN) containing 4 repeats of the immunosuppressive TTAGGG motif commonly found in mammalian telomeric DNA [1].
This ODN was initially identified as a TLR9 antagonist that inhibits immune activation by CpG-containing ODNs [2].
Subsequently, additional targets for this inhibitor were found: by binding to the cytosolic DNA sensors (CDSs) AIM2 and IFI16, A151 prevents AIM2 inflammasome activation [3,4].
This ODN is also described as a cGAS inhibitor, acting through competition with DNA [1].
Overall, these findings show that ODN TTAGGG (A151) is a multiple pattern recognition receptor (PRR) suppressor that can prove useful for immunomodulatory studies.
References:
1. Steinhagen F. et al., 2017. Suppressive oligodeoxynucleotides containing TTAGGG motifs inhibit cGAS activation in human monocytes. Eur J Immunol. DOI: 10.1002/ eji.201747338.
2. Gursel I. et al., 2003. Repetitive elements in mammalian telomeres suppress bacterial DNA-induced immune activation. J Immunol. 171(3):1393-400.
3. Eichholz K. et al., 2016. Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells. PLoS Pathog. 12(9):e1005871.
4. Kaminski J. et al., 2013. Synthetic oligodeoxynucleotides containing suppressive TTAGGG motifs inhibit AIM2 inflammasome activation. J Immunol. 191(7):3876-83.
Specifications
Working concentration: 100 nM - 10 µM
Solubility: 5 mg/ml in water
Molecular weight: 7944 g/mol
ODN TTAGGG sequence
5’- tt agg gtt agg gtt agg gtt agg g -3’ (24 mer)
Note: Bases are phosphorothioate-linked (nuclease resistant).
Quality control:
- Biological activity has been tested using HEK-Blue™ TLR9 cells.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
Contents
ODN TTAGGG (A151) is provided lyophilized and is available in two quantities.
tlrl-ttag151:
- 200 μg (25.2 nmol) lyophilized ODN TTAGGG (A151)
- 1.5 ml endotoxin-free water
tlrl-ttag151-1:
- 1 mg (126 nmol) lyophilized ODN TTAGGG (A151)
- 1.5 ml endotoxin-free water
ODN TTAGGG (A151) is shipped at room temperature.
Upon receipt, store at -20 °C.